Molecular mechanisms of transcriptional regulation via histone acetylation

为了理解通过组蛋白修饰进行转录调控的分子机制，我试图从染色质模板中识别转录所需的分子，并一直关注组蛋白乙酰化以分析组蛋白修饰与转录调控之间的关系。使用纯化的体外转录系统，在HeLa核提取物衍生的馏分中鉴定了多种染色质转录激活活性。此外，一些缺乏乙酰化的组蛋白H3或H4突变体表现出与野生型组蛋白不同的转录活性，这表明特定位点的乙酰化对转录调控很重要。

To understand molecular mechanisms of transcriptional regulation via histone modifications, I have tried to identify the molecule(s) that is required for transcription from the chromatin template and have been focusing histone acetylation to analyze the relationship between histone modifications and transcriptional regulation. Using a purified in vitro transcription system, multiple chromatin transcription-enabling activities were identified in HeLa nuclear extract-derived fractions. Also, some of the acetylation-deficient mutants of histone H3 or H4 showed different transcription activity from the wild-type histone, suggesting that acetylation of the specific sites is important for transcriptional regulation.