Mechanistic analyses of transcriptional regulation via RNA-binding proteins

RNA结合蛋白转录调控的机制分析

• 批准号: 23770200
• 负责人: FUKUDA Aya
• 金额: $ 3万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770200/
• 关键词: 転写; コアクチベーター; RNA結合タンパク質; メディエーター; リイニシエーション; 前初期遺伝子; 転写制御

A novel coactivator hnRNP R (heterogeneous nuclear ribonucleoprotein R) facilitates transcription reinitiation from the c-fos promoter in vitro in cooperation with Mediator. The cooperative action of hnRNP R and Mediator is diminished by the CDK8 module, which is comprised of CDK8, Cyclin C, MED12, and MED13 of the Mediator subunits. Consistently, hnRNP R interacts with the Scaffold components (Mediator, TBP, and TFIIH) as well as TFIIB, which recruits Pol II and TFIIF to Scaffold. The RNA transcript produced from the G-free cassette interacts with hnRNP R through its RNA recognition motifs (RRMs) and arginine-glycine-glycine (RGG) domain. Knockdown of hnRNP R in mouse cells compromised rapid induction of several immediate-early genes. These results suggest an important role for hnRNP R in regulating robust response of immediate-early genes.

一种新的辅激活因子hnRNP R（heterogeneous nuclear ribonucleoprotein R）在体外与Mediator协同促进c-fos启动子的转录重起始。hnRNP R和Mediator的协同作用被CDK 8模块减弱，该模块由Mediator亚基的CDK 8、细胞周期蛋白C、MED 12和MED 13组成。因此，hnRNP R与支架组分（介体、TBP和TFIIH）以及TFIIB相互作用，TFIIB将Pol II和TFIIF募集到支架。从无G盒产生的RNA转录物通过其RNA识别基序（RRM）和甘氨酸-甘氨酸-甘氨酸（RGG）结构域与hnRNP R相互作用。敲低小鼠细胞中的hnRNP R损害了几个立即早期基因的快速诱导。这些结果表明hnRNP R在调节即刻早期基因的强烈反应中具有重要作用。

项目成果

Heterogeneous nuclear ribonucleoprotein R (hnRNP R) cooperates Mediator to enhance transcription reinitiation

异质核核糖核蛋白 R (hnRNP R) 与介体合作增强转录重新启动

• 发表时间: 2011
• 作者: Fukuda A;Shimada M;Nakadai T;Nishimura K;Hisatake K
• 通讯作者: Hisatake K

筑波大学遺伝子制御学研究室

筑波大学基因调控实验室

The RNA-binding protein complexes NF45/90 and NF45/110 activate c-fos transcription in a signal-dependent way

RNA 结合蛋白复合物 NF45/90 和 NF45/110 以信号依赖性方式激活 c-fos 转录

• 发表时间: 2012
• 作者: Ochi A;Makabe K;Yamagami R;Hirata A;Sakaguchi R;Hou YM;Watanabe K;Nureki O;Kuwajima K;Hori H;Fukuda A
• 通讯作者: Fukuda A

筑波大学医学医療系 遺伝子制御学研究室ホームページ

筑波大学医学部基因调控实验室主页

Heterogeneous nuclear ribonucleoprotein R (hnRNP R) cooperates with Mediator to enhance transcription reinitiation

异质核核糖核蛋白 R (hnRNP R) 与 Mediator 合作增强转录重新启动

• 发表时间: 2011
• 作者: Eisuke Takao;Eri Chatani;Hiroshi Imamura;and Minoru Kato;Yumiko Ohhashi and Motomasa Tanaka;Fukuda A
• 通讯作者: Fukuda A