Novel pyrethroid-metabolizing enzyme in the silkmoth

谷胱甘肽转移酶是解毒酶的一个主要家族。在这里，我们报告的底物特异性，未分类的GST的家蚕，bmGSTU的晶体结构，并显示它发生作为一个二聚体，像其他GST。bmGSTU能够将谷胱甘肽缀合至GST的通用底物1-氯-2，4-二硝基苯。bmGSTU的结构用2. 1A，通过同步辐射和分子置换方法。bmGSTU的每个亚基在活性中心都有一个谷胱甘肽结合位点。此外，bmGSTU mRNA在B耐药株中也有高表达。森。

Glutathione transferases comprise a major family of detoxification enzyme. Here, we report the substrate specificity, the crystal structure of unclassified GST of Bombyx mori, bmGSTU, and showing that it occurred as a dimer, like other GSTs. The bmGSTU was able to conjugate glutathione to 1-chloro-2, 4-dinitrobenzene, the universal substrate of GST. The structure of bmGSTU was determined with resolutions of 2. 1 A, by synchrotron radiation and molecular replacement method. Each subunit of bmGSTU displayed a glutathione-binding site in the active center. Furthermore, the bmGSTU mRNA was highly expressed in insecticide-resistance strain of B. mori.