Development of delivery systems of functional food factors by vesiculation and modulation of transepithelial transport

通过囊泡化和跨上皮运输调节开发功能性食品因子的输送系统

• 批准号: 21780138
• 负责人: WATANABE Jun
• 金额: $ 3万
• 依托单位: National Agricultural Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780138/
• 关键词: ナノスケール化; 細胞内輸送; フラボノイド; Caco-2細胞; ヘスペレチン; ベシクル

In this research, we aimed to enhance the absorption of functional food factors, especially flavonoids, by optimization of vasiculation condition of flavonoids and by modulation of transepithelial transport in the intestine. When flavonoids were incorporated to vesicles, flavonoids were stably suspended beyond their saturated concentrations, and quercetin was protected from degradation in aqueous media. Permeated hesperetin across the Caco-2 cell monolayer was significantly increased when hesperetin coexisted with lipid vesicles. Several genes related to intracellular transport were down- or up-regulated by the treatment with dipeptide Trp-Gly in Caco-2 cells, suggesting that these genes possibly become the target for modulation of transepithelial transport by food components.

本研究旨在通过优化黄酮类化合物的血管化条件和调节其在肠道内的跨上皮转运，促进功能性食品因子尤其是黄酮类化合物的吸收。当黄酮类化合物被纳入囊泡，黄酮类化合物被稳定地悬浮超过其饱和浓度，槲皮素被保护在水介质中的降解。当橙皮素与脂质囊泡共存时，橙皮素穿过Caco-2细胞单层的渗透显著增加。在Caco-2细胞中，用二肽Trp-Gly处理后，与细胞内转运相关的几个基因下调或上调，这表明这些基因可能成为食物成分调节跨上皮转运的靶点。

项目成果

フラボノイドに対する脂質ベシクルの安定化効果

脂囊泡对黄酮类化合物的稳定作用

• 发表时间: 2011
• 作者: 大泉祐樹;ら
• 通讯作者: ら

脂質ベシクル共存下におけるフラボノイドのCaco-2細胞単層膜透過特性

脂质囊泡存在下黄酮类化合物的 Caco-2 细胞单层膜渗透特性

• 发表时间: 2010
• 作者: 池山明日香;ら
• 通讯作者: ら

Preparation and characterization of lipid vesicles containing a hydrophobic flavonoid

含有疏水性黄酮类化合物的脂质囊泡的制备和表征

• 发表时间: 2009
• 期刊: Journal of Bioscience and Bioengineering 108(S1)
• 作者: Asuka Ikeyama;Maki Kikugawa;Seigo Sato;Jun Watanabe;Sosaku Ichikawa
• 通讯作者: Sosaku Ichikawa