Identification of a factor which improves the differentiation ability of a stem cell

提高干细胞分化能力的因子的鉴定

• 批准号: 21790300
• 负责人: HIKICHI Takafusa
• 金额: $ 2.75万
• 依托单位: Research Institute, International Medical Center of Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790300/
• 关键词: 単為発生; ゲノミックインプリンティング; 核移植; 胎盤形成; 胚発生; 幹細胞

Although siRNA to the gene which the amount of expression increased before and after the nuclear transplantation was introduced into the normal fertilized embryo nature stem cell (ES), respectively, change of the differentiation ability to the nerve cell in in vitro was not observed. And the placentas of parthenogenetic embryos can escape epigenetic regulation when developed using nuclear transfer techniques and can support fetal development to full gestation.

尽管将针对在核移植前后表达量增加的基因的siRNA分别导入正常受精胚胎天然干细胞（ES）中，但未观察到体外向神经细胞分化能力的变化。而孤雌生殖胚胎的胎盘在使用核移植技术发育时可以逃避表观遗传调控，并可以支持胎儿发育到完全妊娠。

项目成果

Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer

• DOI: 10.1242/dev.051375
• 发表时间: 2010-09-01
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Hikichi, Takafusa;Ohta, Hiroshi;Wakayama, Teruhiko
• 通讯作者: Wakayama, Teruhiko