Development of quinone-labeled antibody for non-enzymatic chemiluminescence immunoassay

当对苯二酚与二硫苏糖醇(DTT)和鲁米诺混合时，其化学发光强度与对苯二酚的浓度成正比。因此，我们认为，苯醌标记的抗体可以应用于非酶化学发光免疫分析。本研究通过将1，4-dihydro-1，4-dioxo-3-methyl-2-naphthylmethyl氯甲酸酯偶联到抗体的氨基上，制备了苯醌标记抗体。由于加入DTT和鲁米诺后，醌标记的抗体会发出化学发光，因此它应该成为一种基于抗原-抗体相互作用的新的物质分析工具。

A strong and long-lived chemiluminescence was observed when quinone was mixed with dithiothreitol (DTT) and luminol, and the chemiluminescence intensity was proportional to the concentration of quinone. Therefore, we considered that quinone-labeled antibody could be applied to develop a non-enzymatic chemiluminescence immunoassay. In this study, quinone-labeled antibody was prepared by coupling of 1,4-dihydro-1,4-dioxo-3-methyl-2-naphthylmethyl chloroformate to amino group of antibody. Since the quinone-labeled antibody emitted chemiluminescence after addition of DTT and luminol, it should become a new analytical tool for various substances based on antigen-antibody interaction.