Establishment of the effective cell culture method for regulatory T cells.

建立有效的调节性T细胞培养方法。

• 批准号: 21790535
• 负责人: SHIBAKURA Misako
• 金额: $ 2.58万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790535/
• 关键词: 制御性T細胞; 培養方法; P-セレクチン; PSGL-1; 移植片対宿主病

I tried the establishment of the effective cell culture method of regulatory T cells. To clarify the role of selectins/selectin-ligands in the cell proliferation and the function of Treg cells, we examined the cell proliferation rate and the function of Treg cells, using P-selectin-/-mice and PSGL-1-/-mice. The growth rate of P-selectin-/-Treg cells and PSGL-1-/-Treg cells were high in comparison with the wild-type Treg cells. PSGL-1-/-Treg cells could inhibit wild-type T cell proliferation in in-vitro suppression assay.

尝试建立调节性T细胞的有效细胞培养方法。为了明确选择素/选择素配体在Treg细胞增殖和功能中的作用，我们使用p -选择素-/-小鼠和PSGL-1-/-小鼠检测了Treg细胞的增殖率和功能。与野生型Treg细胞相比，p -选择素-/-Treg细胞和PSGL-1-/-Treg细胞的生长速率较高。在体外抑制实验中，PSGL-1-/- treg细胞可以抑制野生型T细胞的增殖。