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The establishment of the effective culture method of retinal stem / progenitor cells derived from iris tissue and the physiologic function test of differentiated cells

The establishment of the effective culture method of retinal stem / progenitor cells derived from iris tissue and the physiologic function test of differentiated cells
虹膜组织源性视网膜干/祖细胞有效培养方法的建立及分化细胞的生理功能检测
批准号:
22591970
负责人:
YAMAMOTO Naoki
金额:
$2.91万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2012

项目摘要

项目成果

YAMAMOTO Naoki的其他基金

相关文献

中文摘要
翻译
将培养板表面涂以胶原凝胶,培养的小鼠虹膜组织细胞表达组织干细胞标志物。然后,我们通过分选来自培养的人虹膜组织的细胞来有效地培养神经样细胞。当我们在含有视黄酸的营养培养基中培养来自人虹膜组织的组织干/祖细胞时,细胞分化为表达神经视网膜细胞的标记蛋白。应用膜片钳技术检测细胞的钠通道和钾通道的变化。
英文摘要
When we coated the culture plate surface with lamina collagen gel, the culture cells which were derived from mouse iris tissue were expressed the tissue stem cell marker. Then, we were cultured the nerve-like cells effectively by sorting cells derived from culture human iris tissue. When we cultured tissue stem / progenitor cells derived from human iris tissue in a retinoic acid containing nutrient medium, the cells were differentiated the expressed marker protein of the neural retina cell. Furthermore, the physiologic function of cells were evaluated by the patch clamp method, the cells weredetected with a sodium channel and the potassium channel.
期刊论文(0)
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会议论文
DOI: --
发表时间: 2012
期刊: Nihon Jibiinkoka Gakkai Kaiho
影响因子: --
作者: [Kato H, Yui T, Yamamoto N, Okada T, Urano M, Sakurai K, Naito K.]
通讯作者: Naito K.
病気の分子形態学(分担著書)
疾病的分子形态学(合著者)
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [山本直樹, 吉村功, 山本直樹, 山本直樹]
通讯作者: 山本直樹
DOI: --
发表时间: 2012
期刊: AATEX-JaCVAM
影响因子: --
作者: [Kato H, Yui T, Yamamoto N, Okada T, Urano M, Sakurai K, Naito K., 簾内桃子,竹内早苗,増田光輝,山本直樹,小坂忠司,宮岡悦良]
通讯作者: 簾内桃子,竹内早苗,増田光輝,山本直樹,小坂忠司,宮岡悦良
Wnt/beta-catenin signaling regulates melanocyte stem cell differentiation induced by UVB irradiation.
Wnt/β-连环蛋白信号传导调节 UVB 照射诱导的黑素细胞干细胞分化。
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [Yamada T., Hasegawa S., Inoue Y., Date Y., Mizutani H., Yamamoto N., Nakata S., Matsunaga K., Akamatsu H.]
通讯作者: Akamatsu H.
共 18 条
    Regulation of HBs antigen expression in HBV genotype A infection
    The analysis and identification of bome marrow drived liver repair cell
    • 批准号:
      15K09005
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.08万
    • 财政年份:
      2015
    • 负责人:
      YAMAMOTO Naoki
    • 依托单位:
    Development of novel long non-coding RNA mediated DNA methylation editting system
    • 批准号:
      15H06657
    • 项目类别:
      Grant-in-Aid for Research Activity Start-up
    • 资助金额:
      $1.75万
    • 财政年份:
      2015
    • 负责人:
      YAMAMOTO Naoki
    • 依托单位:
    Application of chiral-specificity of amino acids on the novel development of antipsychotic drugs for schizophrenia