Identification of the important factor of GM-CSF sensitized dendritic cells that involves with the process of antitumor effect induced by GM-CSF

GM-CSF致敏树突状细胞参与GM-CSF抗肿瘤作用过程的重要因子的鉴定

• 批准号: 21790773
• 负责人: TANAKA Yoshihiro
• 金额: $ 2.58万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790773/
• 关键词: GM-CSF; 樹状細胞; センダイウイルスベクター; 肺癌; センダイウィルスベクター

To identify the mechanism of antitumor effect induced by s.c. administration of GM-CSF gene transduced LLC (LLC/SeV/GM) cells, we conducted cDNA microarray analysis by using GM-CSF-sensitized DCs harvested from tumor-derived lymph nodes. The result showed that the genes involved in type I interferons-producing DCs (plasmacytoid DCs; pDCs), upon activation, were robustly upregulated in GM-DCs compared with its counterpart. Furthermore, the results of both in vivo depletion of pDCs and s.c. co-administration of TLR7 agonist with LLC/SeV/GM cells indicated that pDCs plays central roles on the antitumor effect induced by GM-CSF.

目的：探讨s.c.诱导抗肿瘤作用的机制。在给予GM-CSF基因转导的LLC（LLC/SeV/GM）细胞后，我们通过使用从肿瘤来源的淋巴结收获的GM-CSF致敏的DC进行cDNA微阵列分析。结果表明，参与I型干扰素产生DC（浆细胞样DC; pDC）的基因在活化后在GM-DC中与其对应物相比强烈上调。此外，pDC的体内消耗和s.c. TLR 7激动剂与LLC/SeV/GM细胞的共施用表明pDC在GM-CSF诱导的抗肿瘤效应中起中心作用。

项目成果

Comparative microarray analysis implicates the pivotal participation of Type I Interferon related signaling pathway in antitumor effect provoked by in vivo GM-CSF gene transduced lung cancer cells

比较微阵列分析表明 I 型干扰素相关信号通路在体内 GM-CSF 基因转导的肺癌细胞引发的抗肿瘤作用中发挥关键作用

• 发表时间: 2011
• 作者: Megumi Narusawa;Hiroyuki Inoue;Yoshihiro Tanaka;Yosuke Yokota;Chika Sakamoto;Shohei Miyamoto;Tomoko Inoue;Makoto Inoue;Koichi Takayama;Mamoru Hasegawa;Yoichi Nakanishi;Kenzaburo Tani
• 通讯作者: Kenzaburo Tani

Comparable transcriptional profile of murine mature dendritic cells in draining lymph node during tumor rejection by non-transmissible Sendai virus encoding GM-CSF infection

编码 GM-CSF 的非传染性仙台病毒感染肿瘤排斥过程中引流淋巴结中小鼠成熟树突状细胞的可比转录谱

• 发表时间: 2010
• 作者: 成澤慈;井上博之;田中芳浩;横田洋介;坂本千香;宮本将平;井上朋子;井上誠;長谷川護;谷憲三朗
• 通讯作者: 谷憲三朗

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