Assessment of the possibility for treating renal anemia by targeting SHP-1.

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To determine whether SHP-1 is a therapeutic target molecule for renal anemia, SHP-1 siRNA induction was performed to knockdown SHP-1 expression in renal anemia model mice. The c-kit positive hematopoietic progenitor cells were isolated from bone marrow of 5/6 nephrectomized mice, and transfection of the c-kit positive hematopoietic progenitor cells with SHP-1 siRNA and shRNA was attempted. Eventually, the experiment was not successful maybe due to the condition for transfection or cell viability.