Analysis of virulence factors of Helicobacter pylori isolates from Vietnam

越南幽门螺杆菌毒力因子分析

• 批准号: 21790961
• 负责人: KURODA Akiko
• 金额: $ 2.75万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790961/
• 关键词: H.pylori; 胃癌; cagA遺伝子; vacA遺伝子; DNA解析; 免疫組織化学; array CGH; 東アジア型CagA; dupA遺伝子

Helicobacter pylori is recognized to play a causative role in the pathogenesis of gastric cancer. The aim of this study was to investigate several genetic factors regarded as virulence or molecular epidemiologic markers in H. pylori isolates from Vietnamese subjects. The cagA, vacA and cag right-end junction genotypes of 103 H. pylori strains from Vietnam were determined by PCR and sequencing. Three types of deletion in the region located upstream of the cagA Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region were identified : the 39-bp deletion type, the 18-bp deletion type, and the no-deletion type. The majority of strains studied (77% ; 80/103) had the 18-bp deletion. The vacA m1 genotype was significantly more common in strains isolated in Hanoi, where the incidence of gastric cancer is higher, than in strains from Ho Chi Minh.

幽门螺杆菌在胃癌的发病机制中起重要作用。本研究的目的是探讨几个遗传因素作为毒力或分子流行病学标记在H。pylori分离物。103株H.通过PCR和测序确定来自越南的pylori菌株。在cagA Glu-Pro-Ile-Tyr-Ala（EPIYA）重复区上游的区域中鉴定了三种类型的缺失：39-bp缺失型、18-bp缺失型和无缺失型。研究的大多数菌株（77% ; 80/103）都有18-bp缺失。vacA m1基因型在胃癌发病率较高的河内分离的菌株中明显比来自胡志明的菌株更常见。

项目成果

Genomic profiling of submucosal-invasive gastric cancer by array-based comparative genomic hybridization.

• DOI: 10.1371/journal.pone.0022313
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kuroda A;Tsukamoto Y;Nguyen LT;Noguchi T;Takeuchi I;Uchida M;Uchida T;Hijiya N;Nakada C;Okimoto T;Kodama M;Murakami K;Matsuura K;Seto M;Ito H;Fujioka T;Moriyama M
• 通讯作者: Moriyama M

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Genomic profiling of gastric carcinoma in situ and adenomas by array-based comparative genomic hybridization

• DOI: 10.1002/path.2686
• 发表时间: 2010-05-01
• 期刊: JOURNAL OF PATHOLOGY
• 影响因子: 7.3
• 作者: Uchida, Masahiro;Tsukamoto, Yoshiyuki;Moriyama, Masatsugu
• 通讯作者: Moriyama, Masatsugu

Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population.

• DOI: 10.1186/1471-2180-9-175
• 发表时间: 2009-08-23
• 期刊: BMC microbiology
• 影响因子: 4.2
• 作者: Uchida T;Nguyen LT;Takayama A;Okimoto T;Kodama M;Murakami K;Matsuhisa T;Trinh TD;Ta L;Ho DQ;Hoang HH;Kishida T;Fujioka T;Moriyama M;Yamaoka Y
• 通讯作者: Yamaoka Y

Evaluation of the Anti-East Asian CagA-Specific Antibody for CagA Phenotyping

• DOI: 10.1128/cvi.00200-09
• 发表时间: 2009-11-01
• 期刊: CLINICAL AND VACCINE IMMUNOLOGY
• 作者: Lam Tung Nguyen;Uchida, Tomohisa;Moriyama, Masatsugu
• 通讯作者: Moriyama, Masatsugu