Regulatory factor on binding of Desmoglein3 and p120ctn during blister formation in pemphigus

天疱疮水疱形成过程中 Desmoglein3 和 p120ctn 结合的调控因素

• 批准号: 21791070
• 负责人: SHU En
• 金额: $ 2.83万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791070/
• 关键词: 皮膚病理学; 天疱瘡; デスモグレイン3; P120カテニン; p120カテニン

In order to elucidate the signal pathway of blister formation in pemphigus, we examined the phosphorylation of pl2Octn in keratinocytes treated with pemphigus-IgG.(1) We performed immunofluorescence staining with DJM-1 cells treated with pemphigus-IgG, however, phospho-p120ctn were not observed.(2) We stimulated normal human keratinocytes and subjected those cells to immunoprecipitation. There are no significant differences in phosphorylation of p120ctn between untreated cells, cells treated with pemphigus and normal human IgG.

为了阐明天疱疮中水疱形成的信号通路，我们检测了用天疱疮-IgG处理的角质形成细胞中pl 2 Octn的磷酸化。(1)我们用天疱疮-IgG处理的DJM-1细胞进行免疫荧光染色，然而，没有观察到磷酸化p120 ctn。(2)我们刺激正常人角质形成细胞，并对这些细胞进行免疫沉淀。未处理的细胞、天疱疮处理的细胞和正常人IgG之间p120 ctn的磷酸化没有显著差异。