Effects of LECT2 on chondrocyte differentiation

LECT2对软骨细胞分化的影响

• 批准号: 21791422
• 负责人: OKUMURA Akinori
• 金额: $ 2.5万
• 依托单位: Research Institute, International Medical Center of Japan (2010-2011)Nihon University (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791422/
• 关键词: 骨・軟骨代謝学; LECT2; 成長板軟骨; 蛋白質; 発生・分化

Regulatory mechanisms of chondrocyte differentiation in the growth plate are still not fully understood. In this study, we analyzed effects of LECT2 on growth plate chondrocyte differentiation using Lect2-deficient mice and a prechondrocytic cell line. Histological analysis revealed that Lect2-KO mice have slightly abnormal growth plate structure. DNA microarray analysis shows that addition of LECT2 leads to changes in factors of chondrocyte differentiation in prechondrocytic cell line. These results suggest that LECT2 might participate in the differentiation of growth plate chondrocyte.

生长板中软骨细胞分化的调节机制仍不完全清楚。在这项研究中，我们分析了LECT 2对生长板软骨细胞分化的影响，使用Lect 2缺陷小鼠和前软骨细胞系。组织学分析显示Lect 2-KO小鼠具有轻微异常的生长板结构。DNA微阵列分析显示，LECT 2的加入导致前软骨细胞系中软骨细胞分化因子的改变。这些结果表明，LECT 2可能参与了生长板软骨细胞的分化。

项目成果

ガラクトサミン/LPS肝障害モデルを用いたLECT2の機能解析

使用半乳糖胺/LPS肝损伤模型分析LECT2的功能

• 发表时间: 2009
• 作者: 奥村彰規;大川原明子;山越智
• 通讯作者: 山越智

Identification and assignment of three disulfide bonds in mammalian leukocyte cell-derived chemotaxin 2 by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

通过基质辅助激光解吸/电离飞行时间质谱法鉴定和分配哺乳动物白细胞来源的趋化素 2 中的三个二硫键

• 发表时间: 2009
• 期刊: BioScience Trends
• 影响因子: 5.5
• 作者: Akinori Okumura;Takehiro Suzuki;Naoshi Dohmae;Tomoya Okabe;Yuki Hashimoto;Katsuyoshi Nakazato;Hideaki Ohno;Yoshitsugu Miyazaki;Satoshi Yamagoe
• 通讯作者: Satoshi Yamagoe