production of monoclonal antibodies against prostate cancer stem cells using differential dendritic cell immunization

将表达HA抗原的调节性树突状细胞免疫小鼠。与正常树突状细胞相比，抗HA抗体的诱生较少。然而，HA免疫后的OVA蛋白免疫显示，抗OVA抗体的诱导类似，提示不同的树突状细胞免疫方案是正确的。树突状细胞吞噬从培养细胞中提取的膜蛋白。将这些树突状细胞免疫小鼠。培养细胞产生抗细胞表面蛋白，但在调节性树突状细胞中较少。

The regulatory dendritic cells expressing HA antigen were immunized into mice. The anti-HA antibody was less induced compared with normal dendritic cells. However, OVA protein immunization after HA immunization demonstrated anti-OVA antibody was similarly induced, suggesting differential dendritic cell immunization protocol worked properly. Dendritic cells were shown to phagocyte the membrane proteins extracted from cultured cells. These dendritic cells were immunized into mice. Anti-cell surface proteins of culture cells were produced, but it was less in the case of regulatory dendritic cells.