production of monoclonal antibodies against prostate cancer stem cells using differential dendritic cell immunization

使用差异树突细胞免疫生产抗前列腺癌干细胞的单克隆抗体

• 批准号: 21791532
• 负责人: TAMURA Takahiko
• 金额: $ 2.25万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791532/
• 关键词: 腫瘍学; 抗体作製; 樹状細胞; 抗体; 癌幹細胞

The regulatory dendritic cells expressing HA antigen were immunized into mice. The anti-HA antibody was less induced compared with normal dendritic cells. However, OVA protein immunization after HA immunization demonstrated anti-OVA antibody was similarly induced, suggesting differential dendritic cell immunization protocol worked properly. Dendritic cells were shown to phagocyte the membrane proteins extracted from cultured cells. These dendritic cells were immunized into mice. Anti-cell surface proteins of culture cells were produced, but it was less in the case of regulatory dendritic cells.

将表达HA抗原的调节性树突状细胞免疫小鼠。与正常树突状细胞相比，抗ha抗体诱导程度较低。然而，HA免疫后的OVA蛋白免疫显示抗OVA抗体的诱导相似，表明差异树突状细胞免疫方案工作正常。树突状细胞可以吞噬从培养细胞中提取的膜蛋白。这些树突状细胞被免疫到小鼠体内。培养细胞产生抗细胞表面蛋白，而调节性树突状细胞产生的抗细胞表面蛋白较少。

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