Steroid metabolizing enzymes in the rat retina : immunohistochemical study.

大鼠视网膜中的类固醇代谢酶：免疫组织化学研究。

• 批准号: 21791706
• 负责人: ITO Takao
• 金额: $ 2.5万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791706/
• 关键词: 網膜; 神経ステロイド; 免疫組織化学; ウエスタンブロット

Neurosteroids are synthesized from cholesterol or other steroidal precursors and affect neuronal functions by modulating neurotransmission in the central nervous system. Recent studies have shown that neurosteroids are produced also in the retina. However, it is unclear where the enzymes are located in the retina and how they contribute to retinal physiology. Here, we investigated the localization of steroid metabolizing enzymes in the developing and adult rat retina by using immunohistochemistry with specific antibodies. Furthermore, to evaluate the developing expression of the enzymes in the retina, we carried out reverse transcription-polymerase chain reaction.

神经类固醇由胆固醇或其他类固醇前体合成，并通过调节中枢神经系统中的神经传递来影响神经元功能。最近的研究表明，神经类固醇也在视网膜中产生。然而，目前还不清楚这些酶在视网膜中的位置以及它们如何促进视网膜生理学。在这里，我们研究了类固醇代谢酶在发育和成年大鼠视网膜的定位，通过使用特异性抗体的免疫组织化学。此外，为了评估视网膜中酶的发育表达，我们进行了逆转录-聚合酶链反应。