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Leukocyte immunophenotyping by a novel method of array system to predict infectious complications in patients with burns or trauma

通过阵列系统的新方法进行白细胞免疫表型分析，预测烧伤或创伤患者的感染并发症

基本信息

批准号：
21791777
负责人：
SEKINE Kazuhiko
金额：
$ 2.91万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791777/
关键词：
外科免疫学臨床外傷・熱傷易感染性

项目摘要

Background and objectives : Critically injured patients are susceptible to multi-organ failure and acute lung injury (ALI) in the presence of mounting infection. Yet no useful and convenient way to clinically predict the progression of organ failure is available, and the role played by the immune response in the gut has yet to be fully characterized. Thus, we conducted clinical research to apply a novel immunophenotyping (IP) method we developed in critically injured or burned patients. Subjects and methods : The novel IP method allows leukocytes fixed to microscope slides in an antigen-specific manner to be observed when a leukocyte suspension is incubated on slides with arrays of leukocyte surface antigens. The method was used for immunophenotyping (CD4, CCR5, CXCR3, CCR4, CRTh2, CD8, CD36 and CD16b) patients with multi-organ failure caused by critical trauma or burn who were treated in our department. Results : IP analysis was conducted in five patients (2 burn patients and 3 trauma patients). In the burn patients expression of the CD4 and CD8 phenotypes was much lower than in healthy adults. Th2 subset (CCR4) expression was particularly suppressed. In the trauma patients, Th1 subset (CCR5, CXCR3) expression was unchanged but Th2 subset (CCR) expression was lower. Discussion : Our IP method enables the comprehensive analysis of leukocyte surface antigens at the bedside of critical burn and trauma patients. We next hope to establish an IP method for clinically predicting organ failure following critical injury and to develop a therapeutic method for preventing organ failure by attenuating exaggerated immune response.

背景和目标：危重创伤患者在感染增加的情况下易发生多器官功能衰竭和急性肺损伤（ALI）。然而，没有有用和方便的方法来临床预测器官衰竭的进展是可用的，肠道免疫反应所起的作用尚未得到充分的表征。因此，我们进行了临床研究，将我们开发的一种新的免疫表型（IP）方法应用于严重受伤或烧伤患者。研究对象和方法：当白细胞悬液在载玻片上与白细胞表面抗原阵列一起孵育时，新的IP方法允许以抗原特异性方式固定到显微镜载玻片上的白细胞被观察到。应用该方法对我科收治的严重创伤或烧伤所致多器官功能衰竭患者进行免疫表型（CD4、CCR5、CXCR3、CCR4、CRTh2、CD8、CD36、CD16b）检测。结果：对5例患者（2例烧伤患者和3例创伤患者）进行了IP分析。烧伤患者的CD4和CD8表型表达明显低于健康成人。Th2亚群（CCR4）表达尤其受到抑制。创伤患者Th1亚群（CCR 5、CXCR 3）表达无变化，Th2亚群（CCR）表达降低。讨论：我们的IP方法可以在危重烧伤和创伤患者的床边全面分析白细胞表面抗原。我们接下来希望建立一种IP方法，用于临床预测严重损伤后的器官衰竭，并开发一种通过减弱过度的免疫反应来预防器官衰竭的治疗方法。