A novel osteoimmunological approach to identify the role of bone resident macrophages and the osteonecrosis of the jaw

在本实验中，双膦酸盐降低了破骨细胞分化过程中的破骨细胞前体的粘附强度。由于抗粘附分子抗体改变破骨细胞的形成，本研究试图寻找整合粘附分子和破骨细胞形成的信号分子。Syk和Fyn是已知对破骨细胞生成重要的酪氨酸激酶，在破骨细胞生成过程中与整合素共免疫沉淀。这些结果表明，由外向内信号整合素是重要的破骨细胞前体功能和分化。

In this experiment bisphosphonates reduced the adherent strength of osteoclast precursors which are in the process of osteoclast differentiation. Because antibody against adhesion molecules altered osteoclastgenesis, I tried to find the signaling molecules which integrate adhesion molecules and osteoclastgenesis. Syk and Fyn, tyrosine kinases known to be important for osteoclastgenesis, were co-immunoprecipitated with integrins in the process of osteoclastgenesis. These results suggest that outside-in signals from integrins are important for osteoclast-precursor function and differentiation.