Study for development of differentiation induction from iPS cell to dental pulp stem cell

虽然在牙科再生医学中开发了用于牙胚再生的治疗方法，但目前还没有涉及可能的临床应用的技术的开发和使用乳牙的牙髓干细胞的应用程度的报道。据报道，可以分化为神经细胞、脂肪细胞、神经细胞、并且在细胞群中具有成骨细胞，其中约1-2%的细胞群存在于具有牙髓干细胞的牙髓中。然而，在这方面，由于细胞数量少，很难应用于实际的生殖治疗。大鼠来源的成釉细胞系作为饲养层，用小鼠来源的iPS细胞成功地在体外将小鼠iPS细胞分化为成釉细胞。通过今后的研究，通过阐明更详细的成釉细胞的产生和分化机制，可以更有效地诱导低风险成釉细胞。结果，并利用新的发现，为牙齿再生药物的临床应用奠定了基础。

It doesn't relate to the development of the technology of a possible clinical application now at once, and extent from which the application of the dental pulp stem cell that uses the deciduous tooth is reported though the treatment method for the tooth germ to reproduce is developed in the odontology department regenerative medicine.It is reported that another differentiative potential that can differentiate into neuronal cell, fat cell, and osteoblast is possessed in the cell population in which about about 1-2% exists in the dental pulp with the dental pulp stem cell. However, it is difficult to apply it to actual reproduction treatment from few of the number of cells.Ameloblast cell line of rat origin as feeder with mouse origin iPS cell succeeded in vittro in differentiated mouse iPS cell into the ameloblast.The inducement of the low risk ameloblast cell is enabled more efficiently by clarifying generation and the differentiation mechanism of a more detailed ameloblast by the research in the future from this result, and using new findings and it goes as base to the clinical applications of regenerative medicines of teeth.