Regeneration of rare birds through germline chimera.

通过种系嵌合体再生珍稀鸟类。

• 批准号: 21658093
• 负责人: KAGAMI Hiroshi
• 金额: $ 2.24万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21658093/
• 关键词: 希少鳥類; 生殖系列キメラ; 代理親; 始原生殖細胞; 生殖キメラ; 鳥類保全; 発生解析; 生殖細胞キメラ; 個体増殖; 遺伝資源保存

PGCs of Gifujidori fowl were collected from the blood of early embryos for transfer to recipients. The embryos were cultured until hatching fertility tests indicated that they had normal reproductive abilities. White Leghorn were used as recipients for chimera production following blood removal. The PGCs were microinjected into the recipients obtaining offspring from Gifujidori. In conclusion, PGCs from the embryos was combined with the conservation of live animals.

从吉富士多利鸡早期胚胎的血液中收集PGCs用于移植给受体。这些胚胎一直被培养，直到孵化的生育能力测试表明它们具有正常的生殖能力。取血后，白来鸿作为嵌合体的受体。将PGCs微注射到从Gifujidori获得后代的受体中。综上所述，胚胎PGCs与活体动物保护相结合。

项目成果

新動物生殖学(佐藤英明編著)

新动物生殖科学（佐藤英明主编）

• 发表时间: 2011
• 作者: 鏡味裕;臼井文武;中村隼明;山本耕裕;大友朝子;柏木まや;田上貴寛;韮澤圭二郎;松原悠子;小野珠乙;下條雅敬;鏡味裕;鏡味裕
• 通讯作者: 鏡味裕

Long Term Culture and Characterization of Chicken Primordial Germ Cells

鸡原始生殖细胞的长期培养和表征

• 发表时间: 2012
• 作者: Miyahara;D.;Nakamura;Y.;Mori;T.;Ono;T.;Nirasawa;K.;IOishi;I.;Tagami;T.;and Kagami;H.
• 通讯作者: H.

Long tem culture and characterization of chicken primordial germ cells

鸡原始生殖细胞的长期培养和表征

• 发表时间: 2012
• 作者: Daichi Miyahars;et al;Hiroshi Kagami
• 通讯作者: Hiroshi Kagami

Germline Replacement by Transfer of Primordial Germ Cells into Partially Sterilized Embryos in the Chicken

• DOI: 10.1095/biolreprod.110.083923
• 发表时间: 2010-07-01
• 期刊: BIOLOGY OF REPRODUCTION
• 影响因子: 3.6
• 作者: Nakamura, Yoshiaki;Usui, Fumitake;Tagami, Takahiro
• 通讯作者: Tagami, Takahiro

Avian Stem Cells for Regeneration of Muscular Dystrophy Chickens by Means of Germline Chimeras

禽干细胞通过种系嵌合体再生肌营养不良鸡

• 发表时间: 2012
• 作者: Fujiwara;A.;Mizutani;M.;Nunooya;T.;Hiramatsu;K.;Ono;T.;and Kagami;H
• 通讯作者: H