Generation and application of gene knock-out rabbits for the study of atherosclerosis

动脉粥样硬化基因敲除兔的培育及应用

• 批准号: 21659078
• 负责人: FAN Jianglin
• 金额: $ 2.16万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21659078/
• 关键词: CETP; 核移植; クローン; HDL; 動脈硬化; ノックアウト; 遺伝子導入; ウサギ; 動脈硬化モデル; 高脂血症; トランスジェニック; モデル動物

It is essential to use genetically modified animals for the elucidation of disease-related gene functions and therapeutic candidate proteins in vivo. In this field, transgenic mice are widely used in the medical research. However, due to the differences between mouse and human in lipid metabolism and cardiovascular system, it is considered that mice are not suitable for the research of lipid metabolism and cardiovascular disease of humans. In the current study, we attempted to establish a somatic cell line with cholesteryl ester transfer protein deficiency and then, using nuclear transfer technology, to make cholesteryl ester transfer protein knock-out rabbits.

在体内阐明与疾病相关的基因功能和治疗性候选蛋白的阐明是必不可少的。在这一领域，转基因小鼠在医学研究中广泛使用。但是，由于脂质代谢和心血管系统中小鼠和人之间的差异，因此认为小鼠不适用于研究人类脂质代谢和心血管疾病的研究。在当前的研究中，我们试图建立具有胆固醇酯转移蛋白缺乏症的体细胞系，然后使用核转移技术使胆固醇转移蛋白转移蛋白敲除兔子。

项目成果

Human C-reactive protein enhances thrombus formation after neaintimal balloon injury in transgenic rabbits.

人 C 反应蛋白增强转基因兔内膜球囊损伤后的血栓形成。

• 发表时间: 2011
• 期刊: J Thromb Haemost.
• 作者: 小熊圭祐;et al.;大島正伸;Ding Y;Pravenec M;大島正伸;大島正伸;Matsuda S
• 通讯作者: Matsuda S

Effects of human C-reactive protein on pathogenesis of features of the metabolic syndrome.

• DOI: 10.1161/hypertensionaha.110.164350
• 发表时间: 2011-04
• 期刊: Hypertension (Dallas, Tex. : 1979)
• 作者: Pravenec M;Kajiya T;Zídek V;Landa V;Mlejnek P;Simáková M;Silhavý J;Malínská H;Oliyarnyk O;Kazdová L;Fan J;Wang J;Kurtz TW
• 通讯作者: Kurtz TW

Endotoxin accelerates atherosclerosis independent of complement activation.

• DOI: 10.1016/j.thromres.2008.06.017
• 发表时间: 2009-02
• 期刊: Thrombosis research
• 影响因子: 7.5
• 作者: K. Reifenberg;H. Lehr;Jianglin Fan;T. Koike;Elena Wiese;Ines Küpper;T. Sagban;S. Schaefer;U. Zähringer;M. Torzewski;K. Lackner;S. Bhakdi

Human C-reactive protein does not promote atherosclerosis in transgenic rabbits.

• DOI: 10.1161/circulationaha.109.872796
• 发表时间: 2009-11-24
• 期刊: Circulation
• 影响因子: 37.8
• 作者: Koike T;Kitajima S;Yu Y;Nishijima K;Zhang J;Ozaki Y;Morimoto M;Watanabe T;Bhakdi S;Asada Y;Chen YE;Fan J
• 通讯作者: Fan J

Hypertriglyceridemia and delayed clearance of fat load in transgenic rabbits expressing human apolipoprotein CIII

• DOI: 10.1007/s11248-010-9467-5
• 发表时间: 2011-08-01
• 期刊: TRANSGENIC RESEARCH
• 影响因子: 3
• 作者: Ding, Yinyuan;Wang, Yuhui;Liu, Enqi
• 通讯作者: Liu, Enqi