Analysis of the novel sialylated N-glycan expressed in the mouse brain

对糖基转移酶基因敲除小鼠和先天性糖基化障碍的分析表明，N-糖链在大脑中扮演着重要的角色。在这项研究中，我们开发了一种简单的方法来纯化和检测微量的N-葡聚糖。我们重点分析了在小鼠脑中发现的一种新的唾液酸化N-糖链结构，6-唾液酸基-路易斯^c(Galβ1，3[NeuAcα2，6]GlcNAc-)。我们的目标是确定其相互作用的分子以及它所依赖的蛋白质。

Analyses of glycosyltransferase knockout mice and congenital disorders of glycosylation reveal that N-glycans play important roles in the brain. In this study, we developed a simple method to purify and detect trace amounts of N-glycans. We focused on the analysis of the novel sialylated N-glycan structure, 6-Sialyl-Lewis^c(Galβ1, 3[NeuAcα2, 6] GlcNAc-), found in the mouse brain. We aimed to identify its interacting molecules as well as the protein on which it is beared.