The function of cerebellar nucleus and cortex in eyeblink conditioning

条件性眨眼是典型的小脑依赖性运动学习。小脑皮层和间位核（IN）分别通过苔藓纤维和攀爬纤维投射接收条件刺激（CS）和非条件刺激（US）信息。然而，联想眨眼学习的潜在机制尚不清楚。为了分析参与条件反应习得（CR）的in的神经可塑性，我在体内记录了眨眼条件反射过程中的神经活动。未训练眨眼条件反射的小鼠脑内部分神经元仅对US有反应，活动增加。另一方面，训练小鼠的IN神经元对US和CS的反应均增加。为了分析CR的详细时间信息，我们使用高速摄像机观察眼睑运动。训练过眨眼条件反射的小鼠对CS的反应更大。小脑皮质病变使CR的潜伏期小而短。

Conditioned eyeblink is typical cerebellum-dependent motor learning. The cerebellar cortex and the interpositus nucleus (IN) receive both conditioned stimulus (CS) and unconditioned stimulus (US) information through the mossy fiber and climbing fiber projections, respectively. However, the underlying mechanism of associative eyeblink learning remains unclear.To analyze neural plasticity in the IN involved in acquisition of conditioned response (CR), I recorded neural activity during eyeblink conditioning in vivo. Some neurons in the IN of mice which have not trained eyeblink conditioning showed increased activities respond only to US. On the other hand, IN neurons of trained mice showed increased activities respond to both US and CS.To analyze the detail temporal information of CR, we observed the eyelid movement using high-speed video camera. Mice which have trained eyeblink conditioning showed larger eyelid closure respond to CS. Lesion of cerebellar cortex made CR to small and short latency response.