The function of cerebellar nucleus and cortex in eyeblink conditioning

小脑核和皮质在眨眼调节中的功能

• 批准号: 21700439
• 负责人: YAWATA Satoshi
• 金额: $ 2.83万
• 依托单位: 財団法人大阪バイオサイエンス研究所 (2010)Osaka Bioscience Institute (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700439/
• 关键词: ニューロン; シナプス; 神経回路

Conditioned eyeblink is typical cerebellum-dependent motor learning. The cerebellar cortex and the interpositus nucleus (IN) receive both conditioned stimulus (CS) and unconditioned stimulus (US) information through the mossy fiber and climbing fiber projections, respectively. However, the underlying mechanism of associative eyeblink learning remains unclear.To analyze neural plasticity in the IN involved in acquisition of conditioned response (CR), I recorded neural activity during eyeblink conditioning in vivo. Some neurons in the IN of mice which have not trained eyeblink conditioning showed increased activities respond only to US. On the other hand, IN neurons of trained mice showed increased activities respond to both US and CS.To analyze the detail temporal information of CR, we observed the eyelid movement using high-speed video camera. Mice which have trained eyeblink conditioning showed larger eyelid closure respond to CS. Lesion of cerebellar cortex made CR to small and short latency response.

条件性眨眼是典型的小脑依赖性运动学习。小脑皮质和间位核分别通过苔藓纤维和攀爬纤维投射接受条件刺激和非条件刺激信息。然而，联合眨眼学习的潜在机制尚不清楚，为了分析条件反应（CR）获得过程中IN的神经可塑性，我记录了在体眨眼条件反射过程中的神经活动。在没有训练过眨眼条件反射的小鼠的IN中，一些神经元显示出仅对US有反应的活动增加。另一方面，训练小鼠的IN神经元对US和CS均表现出增加的活动。为了分析CR的详细时间信息，我们使用高速摄像机观察眼睑运动。训练过眨眼条件反射的小鼠对CS表现出更大的眼睑闭合反应。小脑皮质损伤使CR反应小、潜伏期短。

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