Study on the skeletal muscle derived bioactive substances induced by muscle contraction

肌肉收缩引起的骨骼肌来源生物活性物质的研究

• 批准号: 21700657
• 负责人: MANABE Yasuko
• 金额: $ 2.83万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700657/
• 关键词: 骨格筋; 筋収縮; ミオカイン; プロテオミクス; 分泌蛋白質

In this study, a novel muscle contraction system to explore the muscle derived bioactive substances induced by muscle contraction was developed. C2C12 cells were cultured in the 10%FBS, followed by differentiation in the 2% CS differentiation medium for the 5 days. Differentiated C2C12myotubes were then contracted by a specific contraction system equipped with the carbon electrode. The medium was concentrated and candidate myokines were identified by proteomic analyses.

本研究开发了一种新型的肌肉收缩系统，以探索肌肉收缩诱导的肌源性生物活性物质。将C2C12细胞在10%FBS中培养，然后在2%CS分化培养基中分化5天。然后通过配备有碳电极的特定收缩系统使分化的C2C12肌管收缩。浓缩培养基，通过蛋白质组学分析鉴定候选肌因子。

项目成果

筋収縮時の糖取り込みを調節する細胞内情報伝達経路. 連載;運動生理学分野の先端研究

肌肉收缩过程中调节葡萄糖摄取的细胞内信号转导途径：运动生理学领域的高级研究。

• 发表时间: 2009
• 期刊: 体育の科学 59
• 作者: 眞鍋康子;豊田太郎;藤井宣晴.
• 通讯作者: 藤井宣晴.

Development of the cultured-muscle cell (C2C12) contraction systemby electric stimulation

通过电刺激开发培养肌肉细胞（C2C12）收缩系统

• 发表时间: 2011
• 作者: Manabe Y;Miyatake S;Okeda A;Nakano T;Hirshman MF;Goodyear LJ;Fujii N
• 通讯作者: Fujii N

Intragastric infusion of glucose enhances the rewarding effect of sorbitol fatty acid ester ingestion as measured by conditioned place preference in mice

• DOI: 10.1016/j.physbeh.2009.12.018
• 发表时间: 2010-03-30
• 期刊: PHYSIOLOGY & BEHAVIOR
• 影响因子: 2.9
• 作者: Matsumura, Shigenobu;Yoneda, Takeshi;Fushiki, Tohru
• 通讯作者: Fushiki, Tohru

Taylor and Francis Group, Fat Detection(J.P.Montmayeur, J.Coutre (eds))(Fat Detection : taste, texture, and post ingestive effects)

Taylor 和 Francis Group，脂肪检测（J.P.Montmayeur、J.Coutre（编辑））（脂肪检测：味道、质地和摄入后影响）

• 发表时间: 2009
• 作者: Manabe Y.;Matsumura S.;Fushiki T
• 通讯作者: Fushiki T

Goodyear, Nobuharu Fujii Development of the cultured-muscle cell (C2C12) contraction system by electric stimulation

Goodyear、Nobuharu Fujii 通过电刺激开发培养肌肉细胞 (C2C12) 收缩系统

• 发表时间: 2011
• 作者: Yasuko Manabe;Syouta Miyatake;Ai Okeda;Taemi Nakano;Michael M. Hirshman*;Laurie J.
• 通讯作者: Laurie J.