Molecular mechanism of the emission of flower volatiles synthesized through non-mevalonic acid pathway

为了获得对芳香花的育种有用的知识，使用切花进行以下实验。检测到香茅醇、橙花醇、香叶醇等单萜类化合物、倍半萜类化合物、芳香醇类化合物、乙酸酯类化合物和3，5-二甲氧基甲苯等。这些剪下的玫瑰的香味显示出镇静作用，减少精神疲劳。此外，还克隆了两种单萜类脱氢酶RhMTS 1和RhMTS 2。ThMTS 2被归类为一个由被子植物的无环单萜脱氢酶组成的簇，并且在有香味的品种的紧芽的花瓣中高度表达。另一方面，克隆了两个GPPS基因RhGPPS-LSU 1和RhGPPS 1。RhGPPS-LSU 1在所有香型品种和1个非香型品种的花瓣中表达量较高。

To obtain knowledge useful for the breeding of fragrant flowers, following experiments were conducted using cut roses. Emission of monoterpenes including citronellol, nerol and geraniol, sesquiterpenes, aromatic alcohols, acetate esters and 3,5-dimethoxytoluene were detected. The scent of these cut roses showed a sedative effect and decreased mental fatigue. In addition to these, tow monoterpene synthases, RhMTS1 and RhMTS2, were cloned. The ThMTS2 are grouped into a cluster consisting of acyclic monoterpene synthases of angiosperms and highly expressed in petals of tight buds of scented cultivars. On the other hand, two GPPS genes, RhGPPS-LSU1 and RhGPPS1, were cloned. The expression revel of RhGPPS-LSU1 in petals of all scented cultivars and one non-scented cultivar was high.