Molecular mechanism of the emission of flower volatiles synthesized through non-mevalonic acid pathway

为了获得对香花育种有用的知识，以切花月季为材料进行了以下试验。检测了香茅醇、橙花醇、香叶醇、倍半萜、芳香醇、乙酸酯和3，5-二甲氧基甲苯等单萜类化合物的排放。这些切花玫瑰的香味显示出镇静作用和减轻精神疲劳的作用。此外，还克隆了两种单萜合成酶RhMTS1和RhMTS2。ThMTS2是由被子植物的无环单萜合酶组成的簇，在香型品种紧凑花蕾的花瓣中高度表达。另一方面，克隆了两个GPPS基因RhGPPS-LSU1和RhGPPS1。RhGPPS-LSU1在所有香型品种和1个非香型品种的花瓣中都有较高的表达水平。

To obtain knowledge useful for the breeding of fragrant flowers, following experiments were conducted using cut roses. Emission of monoterpenes including citronellol, nerol and geraniol, sesquiterpenes, aromatic alcohols, acetate esters and 3,5-dimethoxytoluene were detected. The scent of these cut roses showed a sedative effect and decreased mental fatigue. In addition to these, tow monoterpene synthases, RhMTS1 and RhMTS2, were cloned. The ThMTS2 are grouped into a cluster consisting of acyclic monoterpene synthases of angiosperms and highly expressed in petals of tight buds of scented cultivars. On the other hand, two GPPS genes, RhGPPS-LSU1 and RhGPPS1, were cloned. The expression revel of RhGPPS-LSU1 in petals of all scented cultivars and one non-scented cultivar was high.