Molecular and physiological studies on some ectomycorrhizal symbiotic fungi

本研究以日本外生菌根真菌为研究对象，包括松茸(Tricholoma Matsutaka)、松茸(T.bakamatsutake)(松茸与松茸的同种)、石杉(Lyophylum shimeji.)和十字松茸(L.decastes)(与松茸的腐生亲缘关系密切，亲缘关系密切)。通过单次担孢子分离获得了这些真菌的单倍体菌株，比较了它们在菌落生长和外生菌根形成等方面的表型，并测试了这些真菌种对农业侵染的能力。最后，我们选择了一个具有代表性的单倍体品系，分别为石美鸡和十倍体。用HiSeq2000测序系统进行基因组测序。

In this study, Japanese ectomycorrhizal fungi, e.g. Tricholoma matsutake， T.bakamatsutake(an allies of T. matsutakewith different host spectrum)，Lyophylum shimejiand L. decastes(saprophytic and phylogenetically closely related species of L. shimeji) were featured. We isolated haploid strains of these fungal species bysingle basidiospore isolations, and compared their phenotypes on colonial growth and ectomycorrhizal formations etc. We also tested competency of these fungal species for Agro-infections. Finally, we chose a representative haploid strain of L. shimejiand L. decastes, respectively. The genomes were sequenced using HiSeq2000 system.