Generation of clock oscillators in bovine endometrial cells and their functions

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This study was performed to evaluate the roles of circadian clockwork in bovine uterus stromal andepithelial cells during the stages of estrous cycle and pregnancy, compared to the rat uterus cells.To analyze clock-controlled genes, we used siRNA against a core clock gene Bmal1 and an agonistor an antagonist to Rev-erbα. In the Bmal1-knockdowned stromal cells, Rev-erbα and Ptgs2expression were decreased. However, the Ptgs2 gene was increased with Rev-erbα antagonistSR8278, but not its agonist heme, in both cell types. These results strongly suggest that thesynthesis of prostaglandin is controlled under circadian clockwork