Generation of clock oscillators in bovine endometrial cells and their functions

本研究以大鼠子宫细胞为对照，研究了生物钟在发情周期和妊娠期间牛子宫间质上皮细胞中的作用。为了分析时钟控制的基因，我们使用了针对核心时钟基因BMal1的小干扰RNA和针对Rev-erbα的激动剂。在BMal1基因敲除的基质细胞中，Rev-erbα和Ptgs2的表达降低。然而，在两种细胞类型中，REV-ERBα拮抗剂SR8278增加了PTGS2基因，而其激动剂血红素却没有。这些结果有力地表明前列腺素的合成受昼夜节律的控制。

This study was performed to evaluate the roles of circadian clockwork in bovine uterus stromal andepithelial cells during the stages of estrous cycle and pregnancy, compared to the rat uterus cells.To analyze clock-controlled genes, we used siRNA against a core clock gene Bmal1 and an agonistor an antagonist to Rev-erbα. In the Bmal1-knockdowned stromal cells, Rev-erbα and Ptgs2expression were decreased. However, the Ptgs2 gene was increased with Rev-erbα antagonistSR8278, but not its agonist heme, in both cell types. These results strongly suggest that thesynthesis of prostaglandin is controlled under circadian clockwork