Immunomodulatory effects ofB cell derivedVEGF-A in mice

B细胞来源的VEGF-A对小鼠的免疫调节作用

• 批准号: 22390179
• 负责人: HASHIGUCHI Teruto
• 金额: $ 11.9万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390179/
• 关键词: 神経; 免疫; VEGF; リンパ節; B細胞; 高内皮小静脈; LPS tolerance; B cell; immune; lymph; spleen; iron; HEV; angiogenesis; VEGF-A; POEMS syndrome; immune system; nervus system; lymph node; high endothelial venule; mouse

Vascular endothelial growth factor A (VEGF-A) is a prominent growth factor for both angiogenesis and lymphangiogenesis. Recentstudies have shown the importance of VEGF-A in enhancing the growth of lymphatic endothelial cells in lymph nodes (LNs) and the migration of dendritic cells into LNs. VEGF-A is produced in inflamed tissues and/or in draining LNs, where B cells are a possible source of this growth factor. To study the effect of B cell-derived VEGF-A, we created transgenic mice (CD19Cre/hVEGF-Afl) that express human VEGF-A specifically in B cells. We found that the human VEGF-A produced by B cells not only induced lymphangiogenesis in LNs, but also induced the expansion of LNs and the development of high endothelial venules. Contrary to our expectation, we observed a significant decrease in the Ag-specific Ab production postimmunization with OVA and in the proinflammatory cytokine production postinoculation with LPS in these mice. Our findings suggest immunomodulatory effects of VEGF-A: B cell-derived VEGF-A promotes both lymphangiogenesis and angiogenesis within LNs, but then suppresses certain aspects of the ensuing immune responses. 交付決定額

血管内皮生长因子A（VEGF-A）是血管生成和淋巴发生的重要生长因子。最近的研究表明，VEGF-A在增强淋巴结淋巴结（LNS）中淋巴内皮细胞的生长以及树突状细胞迁移到LNS中的重要性。 VEGF-A在燃烧组织和/或排水LN中产生，其中B细胞是该生长因子的可能来源。为了研究B细胞衍生的VEGF-A的效果，我们创建了专门在B细胞中表达人VEGF-A的转基因小鼠（CD19CRE/HVEGF-AFL）。我们发现，B细胞产生的人VEGF-A不仅诱导LNS中的淋巴管生成，而且还诱导了LNS的膨胀和高内皮静脉的发展。与我们的预期相反，我们观察到在这些小鼠中与LPS在LPS接种后的促进性细胞因子生产中，Ag特异性的AB生产量显着下降。我们的发现表明，VEGF-A：B细胞衍生的VEGF-A的免疫调节作用促进了LNS内的淋巴接种和血管生成，但随后抑制了随后的免疫响应的某些方面。赠款决定的数量

项目成果

Intravitreous VEGF-A in eyes with massive vitreous hemorrhage

• DOI: 10.1007/s00417-011-1795-5
• 发表时间: 2011-12-01
• 期刊: GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
• 影响因子: 2.7
• 作者: Shirasawa, Makoto;Arimura, Noboru;Sakamoto, Taiji
• 通讯作者: Sakamoto, Taiji

Effectiveness of esophagus detection by three-dimensional electroanatomical mapping to avoid esophageal injury during ablation of atrial fibrillation.

通过三维电解剖测绘检测食管的有效性，以避免房颤消融过程中食管损伤。

• DOI: 10.1016/j.jjcc.2012.02.009
• 发表时间: 2012
• 期刊: J Cardiol
• 影响因子: 2.5
• 作者: Horie S;Yamasaki T;Okamoto T;Kan S;Ogata K;Miyauchi S;Tobimatsu S.;Maenosono R
• 通讯作者: Maenosono R

Highly sensitive lens culinaris agglutinin-reactive α-fetoprotein is useful for early detection of hepatocellular carcinoma in patients with chronic liver disease

• DOI: 10.3892/or.2011.1425
• 发表时间: 2011-11-01
• 期刊: ONCOLOGY REPORTS
• 影响因子: 4.2
• 作者: Oda, Kohei;Ido, Akio;Tsubouchi, Hirohito
• 通讯作者: Tsubouchi, Hirohito

Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device-related infections

• DOI: 10.1111/j.1574-695x.2011.00821.x
• 发表时间: 2011-10-01
• 期刊: FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
• 作者: Kawamura, Hideki;Nishi, Junichiro;Komiya, Setsuro
• 通讯作者: Komiya, Setsuro

MK615, a Prunus mume Steb. Et Zucc ('Ume') Extract, Attenuates the Growth of A375 Melanoma Cells by Inhibiting the ERK1/2-Id-1 Pathway

• DOI: 10.1002/ptr.3645
• 发表时间: 2012-06-01
• 期刊: PHYTOTHERAPY RESEARCH
• 影响因子: 7.2
• 作者: Tada, Ko-ichi;Kawahara, Ko-ichi;Kanekura, Takuro
• 通讯作者: Kanekura, Takuro