Immunomodulatory effects ofB cell derivedVEGF-A in mice

B细胞来源的VEGF-A对小鼠的免疫调节作用

• 批准号: 22390179
• 负责人: HASHIGUCHI Teruto
• 金额: $ 11.9万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390179/
• 关键词: 神経; 免疫; VEGF; リンパ節; B細胞; 高内皮小静脈; LPS tolerance; B cell; immune; lymph; spleen; iron; HEV; angiogenesis; VEGF-A; POEMS syndrome; immune system; nervus system; lymph node; high endothelial venule; mouse

Vascular endothelial growth factor A (VEGF-A) is a prominent growth factor for both angiogenesis and lymphangiogenesis. Recentstudies have shown the importance of VEGF-A in enhancing the growth of lymphatic endothelial cells in lymph nodes (LNs) and the migration of dendritic cells into LNs. VEGF-A is produced in inflamed tissues and/or in draining LNs, where B cells are a possible source of this growth factor. To study the effect of B cell-derived VEGF-A, we created transgenic mice (CD19Cre/hVEGF-Afl) that express human VEGF-A specifically in B cells. We found that the human VEGF-A produced by B cells not only induced lymphangiogenesis in LNs, but also induced the expansion of LNs and the development of high endothelial venules. Contrary to our expectation, we observed a significant decrease in the Ag-specific Ab production postimmunization with OVA and in the proinflammatory cytokine production postinoculation with LPS in these mice. Our findings suggest immunomodulatory effects of VEGF-A: B cell-derived VEGF-A promotes both lymphangiogenesis and angiogenesis within LNs, but then suppresses certain aspects of the ensuing immune responses. 交付決定額

Vascular endothelial growth factor A (VEGF-A) is a prominent growth factor for both angiogenesis and lymphangiogenesis. Recent studies have shown the importance of VEGF-A in enhancing the growth of lymphatic endothelial cells in lymph nodes (LNs) and the migration of dendritic cells into LNs. VEGF-A is produced in inflamed tissues and/or in draining LNs, where B cells are a possible source of this growth factor. To study the effect of B cell-derived VEGF-A, we created transgenic mice (CD19Cre/hVEGF-Afl) that express human VEGF-A specifically in B cells. We found that the human VEGF-A produced by B cells not only induced lymphangiogenesis in LNs, but also induced the expansion of LNs and the development of high endothelial venules. Contrary to our expectation, we observed a significant decrease in the Ag-specific Ab production postimmunization with OVA and in the proinflammatory cytokine production postinoculation with LPS in these mice. Our findings suggest immunomodulatory effects of VEGF-A: B cell-derived VEGF-A promotes both lymphangiogenesis and angiogenesis within LNs, but then suppresses certain aspects of the ensuing immune responses. Delivery Determination

项目成果

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• DOI: 10.1007/s00417-011-1795-5
• 发表时间: 2011-12-01
• 期刊: GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
• 影响因子: 2.7
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• 通讯作者: Sakamoto, Taiji

Advanced malignant melanoma responds to Prunus mume Sieb. Et Zucc (Ume) extract: Case report and in vitro study

• DOI: 10.3892/etm_00000089
• 发表时间: 2010-07-01
• 期刊: EXPERIMENTAL AND THERAPEUTIC MEDICINE
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Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device-related infections

• DOI: 10.1111/j.1574-695x.2011.00821.x
• 发表时间: 2011-10-01
• 期刊: FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
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• 通讯作者: Komiya, Setsuro

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• DOI: 10.1016/j.jjcc.2012.02.009
• 发表时间: 2012
• 期刊: J Cardiol
• 影响因子: 2.5
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• 通讯作者: Maenosono R

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• DOI: 10.3892/or.2011.1425
• 发表时间: 2011-11-01
• 期刊: ONCOLOGY REPORTS
• 影响因子: 4.2
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• 通讯作者: Tsubouchi, Hirohito