The elucidation of molecular mechanisms of amelogenesis by epitherial-specific Runx knockout mouse

上皮特异性Runx基因敲除小鼠阐明釉质形成的分子机制

• 批准号: 22390390
• 负责人: HONJO Tadashi
• 金额: $ 12.4万
• 依托单位: Tottori University (2012)Okayama University (2010-2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390390/
• 关键词: Runx1, Cbfb; エナメル芽細胞; 遺伝子改変動物; エナメル質; Runxシグナリング; Runx1; Cbvb; cbfb; runx1; Runx; Cbfb; 歯原性上皮

Targeted deletion of Runx genes has revealed distinct roles for these proteins in development. Here, we report that epithelial Runx genes are involved in regulation of ameloblastdifferentiation. We analyzed the functional role of Cbfb and Runx1 in the enamel formation invivo, using a conditional genetic approach in which the Cbfb and Runx1 gene was inactivated in mouse epithelial cells(K14-Cre/Cbfbfl/fl, K14-Cre/Runx1 fl/fl). We found that mice deficient in epithelial Cbfb and Runx1 resulted in enamel aplasia in the incisors and in hypoplasia in the molars. The present results provide the genetic evidence that Runx1 and Cbfb genes function in the enamel formation in developing molar and incisors.

RUNX基因的靶向缺失揭示了这些蛋白质在发育中的不同作用。在此，我们报道了上皮性RUNX基因参与成釉细胞分化的调控。我们采用在小鼠上皮细胞中Cbfb和RUNX1基因失活的条件遗传学方法(K14-Cre/Cbfbfl/fl，K14-Cre/RUNX1 fl/fl)，分析了Cbfb和RUNX1基因在体内釉质形成中的功能作用。我们发现，缺乏上皮Cbfb和RUNX1的小鼠导致门牙釉质再生不全和磨牙发育不良。这些结果为RUNX1和Cbfb基因在磨牙和门牙发育过程中的釉质形成过程中的作用提供了遗传学证据。

项目成果

Sugawara Y, Kamioka H, Ishihara Y, Fujisawa N, Kawanabe N, Yamashiro T

菅原 Y、神冈 H、石原 Y、藤泽 N、川锅 N、山城 T

• 发表时间: 2013
• 期刊: Bone.
• 作者: Sugawara Y;Kamioka H;Ishihara Y;Fujisawa N;Kawanabe N;Yamashiro T
• 通讯作者: Yamashiro T

Core binding factorβ(Cbfβ) functions in the maintenance of stem cells and orchestrates continuous proliferation and differentiation in mouse incisors

核心结合因子β（Cbfβ）在维持干细胞中发挥作用，并协调小鼠门牙的持续增殖和分化

• 发表时间: 2011
• 作者: Tani;A.;Tada;Y.;Takezawa;T.;Imaizumi;M.;Nomoto;Y.;Nakamura;T.;Omori;K;Yamashiro T
• 通讯作者: Yamashiro T

Microscale fluid flow analysis in a human osteocyte canaliculus using a realistic high-resolution image-based three-dimensional model

• DOI: 10.1039/c2ib20092a
• 发表时间: 2012-01-01
• 期刊: INTEGRATIVE BIOLOGY
• 影响因子: 2.5
• 作者: Kamioka, Hiroshi;Kameo, Yoshitaka;Klein-Nulend, Jenneke
• 通讯作者: Klein-Nulend, Jenneke

Stage-specific embryonic antigen-4 identifies human dental pulp stem cells

• DOI: 10.1016/j.yexcr.2012.01.008
• 发表时间: 2012-03-10
• 期刊: EXPERIMENTAL CELL RESEARCH
• 影响因子: 3.7
• 作者: Kawanabe, Noriaki;Murata, Satoko;Yamashiro, Takashi
• 通讯作者: Yamashiro, Takashi

Cbfbは切歯の上皮幹細胞の維持と、増殖と分化の領域形成に関与する

Cbfb 参与门牙上皮干细胞的维持以及增殖和分化区域的形成。

• 发表时间: 2011
• 作者: Ohno;S.;Hirano;S.;Kanemaru;S.;Kitani;Y.;Kojima;T.;Ishikawa;S.;Mizuta;M.;Tateya;I.;Nakamura;T.;Ito;J.;山城隆
• 通讯作者: 山城隆