Study of nanomaterials on chronic health effect assessment according to endpoint of carcinogenesis and lung fibrosis

纳米材料根据致癌和肺纤维化终点评估慢性健康影响的研究

• 批准号: 22510035
• 负责人: ISHIHARA Yoko
• 金额: $ 2.83万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22510035/
• 关键词: ナノマテリアル; 慢性健康影響; 肺線維化; 発癌; リスク評価; 発がん; ナノ粒子; ミクロ粒子; 動物実験; 慢性暴露; がん遺伝子; 毒性試験; 細胞培養実験; 気管内投与

Experiments were conducted to evaluate properly the chronic effect of nano-materials in vivo and in vitro. Results of cytotoxicity, proliferative activity, mutagenicity and transformation were affected by the physical-chemical properties of test materials in vitro. The risk assessment at concentration of 0.1μg-1mg was performed at the single-dose of tracheal injection in normal animals, and the progress of fibrosis and oncogenesis were observed during 1.6 year in normal animals. Appropriated animal experiment was performed without using genetically modified animals. There were divergence results between in vitro and in vivo experiments.The research results should be carefully applied to human risk assessment.

进行实验以正确评估纳米材料在体内和体外的慢性效应。细胞毒性、增殖活性、致突变性和转化性结果受试验材料的体外理化性质影响。对正常动物单次气管注射进行0.1μg-1mg浓度的风险评估，观察正常动物1.6年内纤维化和肿瘤发生的进展情况。在没有使用转基因动物的情况下进行了适当的动物实验。体外和体内实验结果存在分歧，研究结果应谨慎应用于人体风险评估。

项目成果

Mutagenicity of rare metal and metaloxide nanoparticles: Components and Particles sizes

稀有金属和金属氧化物纳米颗粒的致突变性：成分和颗粒尺寸

• 发表时间: 2012
• 作者: Hasegawa G;Shimonaka M;Ishihara Y
• 通讯作者: Ishihara Y

健康影響と大気中微小粒子状物質

健康影响和大气细颗粒物

• 发表时间: 2012
• 作者: 石原陽子;長谷川豪;中尾元幸;山口みどり
• 通讯作者: 山口みどり

Safty evaluation of fullerene nanowhisker as a new nanoproduct-in vitro experiment

富勒烯纳米晶须作为新型纳米产品的安全性评价——体外实验

• 发表时间: 2010
• 作者: Satoshi Koyama;Go Hasegawa;Yoko Ishihara
• 通讯作者: Yoko Ishihara

Differential gene expression associated with inflammation and blood pressure regulation induced by concentrated ambient particle exposure

• DOI: 10.3109/08958378.2011.625058
• 发表时间: 2011-12-01
• 期刊: INHALATION TOXICOLOGY
• 影响因子: 2.1
• 作者: Hasegawa, Go;Hirano, Masato;Ishihara, Yoko
• 通讯作者: Ishihara, Yoko

Differential genotoxicity of chemical properties and particle size of rare metal and metal oxide nanoparticles

• DOI: 10.1002/jat.1719
• 发表时间: 2012-01-01
• 期刊: JOURNAL OF APPLIED TOXICOLOGY
• 影响因子: 3.3
• 作者: Hasegawa, Go;Shimonaka, Motoyuki;Ishihara, Yoko
• 通讯作者: Ishihara, Yoko