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STUDY OF HEALTH RISK ASSESSMENT OF ENVIRONMENTAL NANO PARTICULATE MATTER BASEDE ON PHYSICAL AND CHEMICAL CARACTERISTICS.

基于理化特性的环境纳米颗粒物健康风险评估研究。

基本信息

批准号：
14580574
负责人：
ISHIHARA Yoko
金额：
$ 2.3万
依托单位：
TOKYO WOMEN'S MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

We studied the particle dosimetry in mice with a single intratracheal injection of nano particulate matter. Particle deposition was observed in type I alveolar epithelial cells and alveolar macrophages in the lung on day 3 after treatment. On day 7, particles were detected in the type I alveolar epithelial cells, basement and blood vessels in the lung. On day 10, particles were detected in the type I cells and blood vessels in the lung, cooper cells in the liver and olfactory. The selection criteria for appropriate makers for the cardiopulmonary diseases-related risk assessment in nano particulate matter were studied in rats exposed with diesel exhaust. The R-R interval, systemic, arrhythmia were selected related to physiological, effects, gene expression of inflammation response-related cytokines, ANP and BNP were selected as for appropriate markers. However, these markers did not show marked correlation with heart failure.Rats treated with particles from diesel exhaust devoid of organic, component showed significant increase of gene expression of inflammation-related cytokines compared with those in rats treated with whole diesel particles. This indicates that the number of nano particles is a major factor for the observed effects rather than their weight.The health effect of organic component of diesel particles was studied in rats using telemetry system. Although inflammatory cells emerged immediately in alveolar spaces after single intratracheal injection of organic components, RR-interval, autonomic nerves system, systemic, and core-temperature did not change markedly for 4 days after treatment.These data suggested that not only the lung where particles initially deposited but also other organs including liver, nerves and brain must be considered in the risk assessment of nano particulate matter. Additionally, physical and chemical characteristics of particles should be factored in the evaluation of health effects of nano particulate matter.

我们研究了小鼠单次气管内注射纳米颗粒物的颗粒剂量学。给药后第3天，在肺中的I型肺泡上皮细胞和肺泡巨噬细胞中观察到颗粒沉积。第7天，在肺的I型肺泡上皮细胞、基底和血管中检测到颗粒。在第10天，在肺中的I型细胞和血管、肝脏和嗅觉中的库珀细胞中检测到颗粒。在接触柴油机尾气的大鼠中研究了纳米颗粒物心肺疾病相关风险评估的合适标记物的选择标准。选择R-R间期、全身性、心律失常、生理效应、炎症反应相关细胞因子基因表达、ANP和BNP作为合适的指标。然而，这些标志物并没有表现出显着的相关性与心力衰竭。大鼠治疗的颗粒从柴油机废气的有机成分缺乏，显示出显着增加的基因表达的炎症相关的细胞因子相比，在大鼠治疗的整个柴油机颗粒。这表明纳米颗粒的数量是观察到的效果的主要因素，而不是他们的重量。使用遥测系统在大鼠中研究柴油颗粒的有机成分的健康效应。虽然在单次气管内注射有机成分后肺泡腔内立即出现炎性细胞，但RR间期、自主神经系统、全身和核心温度在治疗后4天内没有明显变化，这些数据表明，在纳米颗粒物的风险评估中，不仅必须考虑颗粒最初沉积的肺，还必须考虑其他器官，包括肝脏、神经和大脑。此外，在评估纳米颗粒物对健康的影响时，应考虑颗粒物的物理和化学特性。