HEALTH RISK ASSESSMENT OF ENVIRONMENTAL NANO PARTICLES USING MOLECULAR BIOLOGICAL METHODS

利用分子生物学方法评估环境纳米颗粒的健康风险

• 批准号: 16510027
• 负责人: ISHIHARA Yoko
• 金额: $ 2.24万
• 依托单位: KURUME UNIVERSITY (2005-2006)Tokyo Women's Medical University (2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16510027/
• 关键词: Nanoparticles; Risk assessment; health effects; Molecular biology; Ultrafine particles; particle dosimetry; Diesel particulate matters; CAPs; 粒子毒性; DNAプロファイル; サイトカイン; ナノテクノロジー; 微小粒子; DNAファイル; DNA分析; マクロファージ; 健康影響評価

We evaluated the usefulness of molecular biological methods in the health risk assessment of environmental nanoparticles. (1) Lung and heart tissues were collected from aged rats (aged 22-24 months) that had been exposed to diesel particles for 7 months or concentrated air particles (CAPs) for 3 days. The results of DNA profile analysis showed increases in DNA gene expression in complements, macrophage activated cytokine, chemokine, CINC-2, hemoxidase, and angiotensin II receptor in the lung exposed to diesel emission, in complements, inflammation-related components and blood coagulation-related items in the lung exposed to CAPs, and in complements in the heart exposed to CAPs. The increases in DNA expression level did not necessarily correspond with mRNA expression or protein concentration. Physiological and pathological findings and changes in DNA and mRNA expression showed only slight correlations in aged rats exposed to diesel particles or CAPs. On the other hand, the expression of inflammation-related cytokine mRNA was higher in the lungs of rats treated with diesel particles washed with organic solvent (E-DEP) than in those of rats treated with unwashed particles (DEP). E-DEP had a larger particle area per weight than DEP. These findings support the hypothesis that nanoparticles have greater toxicity in the lung than fine particles. The results of our study suggested that DNA profile analysis are useful for the selection of disease-related biomarkers in the first step of the health risk assessment of nanoparticles. Following this, the levels of mRNA expression and protein concentration are confirmed for the selected biomarkers. The final health risk assessment of nanoparticle toxicity should be based on an overall evaluation of molecular biological, biochemical, hematological, pathological and physiological data.

我们评估了分子生物学方法在环境纳米颗粒健康风险评估中的有用性。 (1)从暴露于柴油颗粒7个月或浓缩空气颗粒(CAPs)3天的老年大鼠(22-24月龄)采集肺和心脏组织。 DNA谱分析结果显示，暴露于柴油排放的肺部中的补体、巨噬细胞激活的细胞因子、趋化因子、CINC-2、血红素氧化酶和血管紧张素II受体中的DNA基因表达增加，暴露于CAP的肺部中的补体、炎症相关成分和凝血相关项目以及暴露于CAP的心脏中的补体中的DNA基因表达增加。 DNA 表达水平的增加不一定与 mRNA 表达或蛋白质浓度相对应。在暴露于柴油颗粒或 CAP 的老年大鼠中，生理和病理结果以及 DNA 和 mRNA 表达的变化仅显示出轻微的相关性。另一方面，用有机溶剂洗涤的柴油颗粒（E-DEP）处理的大鼠的肺部炎症相关细胞因子mRNA的表达高于用未洗涤的颗粒（DEP）处理的大鼠的肺部。 E-DEP 的单位重量颗粒面积比 DEP 更大。这些发现支持了这样的假设：纳米粒子在肺中比细颗粒具有更大的毒性。我们的研究结果表明，DNA 谱分析有助于在纳米颗粒健康风险评估的第一步中选择与疾病相关的生物标志物。随后，确认所选生物标志物的 mRNA 表达水平和蛋白质浓度。纳米颗粒毒性的最终健康风险评估应基于分子生物学、生化、血液学、病理学和生理学数据的整体评估。

项目成果

A Nonlinear Analysis of Heart Rate Variability of Rats. Under Diesel Exhaust Emission.

大鼠心率变异性的非线性分析。

• 发表时间: 2006
• 期刊: 生体医工学 第44巻,特別号
• 作者: M Kyoso;M Ishijima;Y Ishihara;K Yana;T Ito;K Maejima
• 通讯作者: K Maejima

Cuteiun in inhibits the proliferation and mineralization of cultured of cultured osteoblasts

Cuteiun 抑制培养成骨细胞的增殖和矿化

• 发表时间: 2006
• 期刊: Eur J Pharmacol 18・534
• 作者: Notoya M;Nishimura H;Woo JT;Nagai K;Ishihara Y;Hagiwara H.
• 通讯作者: Hagiwara H.

Evaluation of Biological Effect by Diesel Exhaust Emission - Analysis of Circadian Rhythm of Heart Rate Variability-

柴油机尾气排放的生物效应评价-心率变异性昼夜节律分析-

• 发表时间: 2005
• 期刊: 生体医工学 第43巻,特別号,PJ3-6-6
• 作者: Y.Takahashi;M.Kyoso;M.Ishijima;Y.Ishihara;K.Yana;T.Ito;K.Maejima
• 通讯作者: K.Maejima

A Nonlinear Analysis of Heart Rate Variability of Rats Under Diesel Exhaust Emission

柴油机尾气排放下大鼠心率变异性的非线性分析

• 发表时间: 2006
• 期刊: Transactions of the Japanese Society for Medical and Biological Engineering 44(suppl.)
• 作者: Kyoso M;Ishijima M;Ishihara Y;Yana K;Ito T;Maejima K
• 通讯作者: Maejima K

Resting R-R interval extraction from R-R histogramto evaluate the effect of diesel emissions.

从 R-R 直方图中提取静息 R-R 区间以评估柴油机排放的影响。

• 发表时间: 2005
• 期刊: Proceeding of 27^<th> Annual Inter.Conf.of the IEEE Engineering In Medicine Biol.Society
• 作者: M Kyoso;M Narisawa;E Ito;M Ishijima;Y Ishihara;K Yana;T Ito;K Maejima
• 通讯作者: K Maejima