Sub-wavelength 3-dimentional imaging of biological tissue

我们建立了一种定量技术来可视化具有微小折射率差异的生物组织的形状和结构。利用自行研制的相位调制微分干涉显微镜，可以得到亚波长的相位分布。此外，当我们观察三维物体时，存在一个问题，即在精确的图像平面之前和之后的模糊图像干扰重建。在此基础上，将最近邻去模糊方法与相位调制微分干涉显微镜的相位重建技术相结合，验证了其有效性。

We established the quantitative technique to visualize the shape and structure of biological tissue which has a small difference of a refractive index. We were able to get the sub-wavelength phase distribution as depth by using the phase modulation differential interference microscope which we developed. Moreover, when we observe three-dimensional object there was a problem which disturbs reconstruction with blurred image before and behind an exact image plane. Consequently, we combined the Nearest Neighbor Deblurring method with the phase reconstruction technique of a phase modulation differential interference microscope, and confirmed its availability.