Effect of excessive folic acid intake on the function and the differentiation of regulatory T cell

过量叶酸摄入对调节性T细胞功能及分化的影响

• 批准号: 22580144
• 负责人: OKAMOTO Yoshihiro
• 金额: $ 3万
• 依托单位: Chiba Institute of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580144/
• 关键词: 食品機能; 免疫寛容; 葉酸; 制御性T細胞; Th17; 制御性Ｔ細胞; 経口免疫寛容

Effect of excessive intake or deficiency of folic acid has not been clarified. It has been reported that the type 4 folic acid receptor (FR4) expresses on the cell surface of regulatory T cell (Treg). An unusual intake of folic acidmay affect the function or differentiation of Treg cell. The excessive intake of folic acid decreased the oral tolerance for anti-ovalbumin (OVA) antibody production, and the deficiency of folic acid enhances oral tolerance for antibody production. An excessive intake or deficiency of folic acid did not affect on naturally occurring Treg (nTreg) population and function. However, the decreased number of induced Treg (iTreg) in mice withexcessive folic acid intake was observed compared with normal mice. On the other hand, the increased number of iTreg was observed in mice with folic acid deficient diet. The plasticity between iTreg and Th17 has been reported. To investigate the effects of folic acid intake on Th17 differentiation, splenocytes were stimulated under Th17-polarizing conditions (immobilized anti-CD3 and anti-CD28 with IL-6 plus TGF-βfor 4 days). The Th17 differentiation enhances with increasing folic acid contents in the diet. The enhanced immune tolerance in mice with folic acid deficient diet might come from the suppressed Th17 differentiation

过量摄入或缺乏叶酸的影响尚未阐明。据报道，4型叶酸受体（FR 4）表达于调节性T细胞（Treg）的细胞表面。叶酸摄入异常可能影响Treg细胞的功能或分化。过量摄入叶酸可降低抗卵清蛋白（OVA）抗体产生的口服耐受性，叶酸缺乏可增强抗体产生的口服耐受性。过量摄入或缺乏叶酸不会影响天然存在的Treg（nTreg）群体和功能。然而，与正常小鼠相比，过量摄入叶酸的小鼠诱导Treg（iTreg）数量减少。另一方面，在叶酸缺乏饮食的小鼠中观察到iTreg数量增加。已经报道了iTreg和Th 17之间的可塑性。为了研究叶酸摄入对Th 17分化的影响，在Th 17极化条件下刺激脾细胞（用IL-6加TGF-β固定抗-CD 3和抗-CD 28 4天）。随着日粮中叶酸含量的增加，Th 17分化增强。叶酸缺乏小鼠免疫耐受的增强可能来自于Th 17分化的抑制

项目成果

Inhibition of interleukin 17 production by curcumin in mice with collagen-induced arthritis

姜黄素抑制胶原诱导关节炎小鼠白细胞介素 17 的产生

• 发表时间: 2011
• 期刊: Biomed Res
• 作者: Yoshihiro Okamoto;Mayuri Tanaka;Takashi Fukui;Toshiyuki Masuzawa
• 通讯作者: Toshiyuki Masuzawa

葉酸過剰摂取の経口免疫寛容誘導に及ぼす影響

过量叶酸摄入对口服免疫耐受诱导的影响

• 发表时间: 2010
• 作者: Tseye-Oidov O;Mikami I;Watanabe;J;Tsushida T;Demberel B;Kimura T;Ide T;岡本能弘
• 通讯作者: 岡本能弘

Brazilian propolis inhibits the differentiation of Th17 cells by inhibition of interleukin-6-induced phosphorylation of signal transducer and activator of transcription 3

• DOI: 10.3109/08923973.2012.657304
• 发表时间: 2012-10-01
• 期刊: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
• 影响因子: 3.3
• 作者: Okamoto, Yoshihiro;Tanaka, Mayuri;Masuzawa, Toshiyuki
• 通讯作者: Masuzawa, Toshiyuki

Suppression of interleukin 17 production by Brazilian propolis in mice with collagen-induced arthritis

• DOI: 10.1007/s10787-011-0088-2
• 发表时间: 2012-02-01
• 期刊: Inflammopharmacology
• 影响因子: 5.8
• 作者: Tanaka, Mayuri;Okamoto, Yoshihiro;Masuzawa, Toshiyuki
• 通讯作者: Masuzawa, Toshiyuki

Brazilian propolis ameliorates trinitrobenzene sulphonic acid-induced colitis in mice by inhibiting Th1 differentiation

巴西蜂胶通过抑制 Th1 分化改善三硝基苯磺酸诱导的小鼠结肠炎

• 发表时间: 2013
• 期刊: Int Immunopharm
• 作者: Yoshihiro Okamoto;Takazumi Hara;Tatsuya Ebato;Takashi Fukui;Toshiyuki Masuzawa
• 通讯作者: Toshiyuki Masuzawa