The verification of the alternative usage hypothesis of the neuro-motor system in teleosts. An attempt in the ayu (Plecoglossus altivelis altivelis) as a model fish by using a molecular marker c-fos.

鲑鱼和ayu Plecoglossus altivelis altivelis也使用躯干肌肉组织进行产卵，但有关的大脑区域尚不清楚。为了获得一种特定的分子工具来确定在产卵的阿鱼调节痉挛性身体收缩的大脑区域，我们首先从阿鱼大脑中分离出c-fos cDNA。基于该序列，我们获得了全长重组ayu c-Fos蛋白和以该蛋白为抗原的特异性抗血清。抗血清免疫染色表明，该脑区可能与中脑运动区对应。

The salmonids and the ayu Plecoglossus altivelis altivelis use the trunk musculature also for spawning, but concerned brain regions have been unknown.In order to obtain a specific molecular tool to determine brain regions regulating spasmodic body contraction in the spawning ayu, we firstly isolated c-fos cDNA from the ayu brain. Based on the sequence, we obtained the full length recombinant ayu c-Fos protein and specific antisera by using the protein as antigens.Immunostaining of brain sections of the spawned ayu with the antiserum suggested that the concerned brain area might correspond to the midbrain locomotor region.