The verification of the alternative usage hypothesis of the neuro-motor system in teleosts. An attempt in the ayu (Plecoglossus altivelis altivelis) as a model fish by using a molecular marker c-fos.

硬骨鱼神经运动系统替代使用假设的验证。

• 批准号: 22580198
• 负责人: UEMATSU Kazumasa
• 金额: $ 3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580198/
• 关键词: アユ; 脳; 産卵中枢; 裸状型卵巣; 体側筋; c-fos; 最初期遺伝子; 特異抗体; 遺伝子発現; タンパク質発現; 抗体作製; 脳地図; クローニング; カイニン酸

The salmonids and the ayu Plecoglossus altivelis altivelis use the trunk musculature also for spawning, but concerned brain regions have been unknown.In order to obtain a specific molecular tool to determine brain regions regulating spasmodic body contraction in the spawning ayu, we firstly isolated c-fos cDNA from the ayu brain. Based on the sequence, we obtained the full length recombinant ayu c-Fos protein and specific antisera by using the protein as antigens.Immunostaining of brain sections of the spawned ayu with the antiserum suggested that the concerned brain area might correspond to the midbrain locomotor region.

鲑科鱼类和香鱼Plecoglossus altivelis altivelis也利用躯干肌肉组织进行产卵，但有关的脑区一直是未知的，为了获得一个特定的分子工具，以确定脑区调节痉挛性身体收缩在产卵香鱼，我们首先从香鱼脑c-fos cDNA。以该蛋白为抗原，获得了香鱼c-Fos全长重组蛋白及其特异性抗血清，免疫组化结果表明，香鱼脑组织可能与中脑运动区有关。