Studies on Cerebellar Mechanisms Underlying Fish Swimming

鱼类游泳的小脑机制研究

• 批准号: 12460086
• 负责人: UEMATSU Kazumasa
• 金额: $ 9.22万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460086/
• 关键词: teleost; cenebellum; swimming; neuron; immunocytochemistry; Purkinje neuron; eurydendroid cell; action potential; キンギョ; 出力ニューロン; 逆行性標識; 破壊; ニューロン活動; 行動; 遊泳運動; 神経伝達物質; 神経連絡; 神経トレーサー; 小脳体; 小脳弁; 神経活動

1. A brain atlas of the goldfish was made for mapping labeled neurons on specific brain structures in this study.2. To identify target brain regions of efferent cerebellar neurons, a neuronal tracer was injected into the corpus cerebelli (CC) and the valvula cerebelli (VC). By injection to CC and medial VC, axon terminals of the cerebellar neurons were found in the contralateral diencephalons, midbrain and medulla, and the ipsilateral VC, including locomotive centers for controlling swimming by either the trunk muscles or the fin.3. Tracer injection into the basal part of CC demonstrated that eurydendroid cells, proper efferent neurons of the teleost fish, were distributed around the Purkinje cell layer. It is the first description that the cells have a few thick primary dendrites expanding on the parasagittal planes of the body and are distributed in the molecular and the granular cell layers.4. It was demonstrated inmunocytochemically for the first time in teleosts that the Purkinje cells are GABAergic and eurydendroid cells are not.5. We developed a method to record electrical activities of cerebellar neurons from swimming fish by using electrodes and an operational amplifier mounted on the head. On the shape of action potentials recorded, we identified cerebellar neurons successfully.6. It is expected to augment the knowledge of cerebellar functions of teleosts by advanced studies based on the present study.

1.本研究制作了金鱼脑图谱，用于标记神经元在特定脑结构上的定位.为了确定传出小脑神经元的靶脑区域，将神经元示踪剂注射到小脑体（CC）和小脑瓣（VC）中。通过注射到CC和内侧VC，在对侧间脑、中脑和延髓以及同侧VC中发现小脑神经元的轴突终末，包括躯干肌或鳍控制游泳的运动中枢.示踪剂注射到CC的基底部表明，广突细胞，硬骨鱼的适当的传出神经元，分布在浦肯野细胞层周围。首次描述了细胞在体部旁平面上有少量粗大的初级树突，分布于分子细胞层和颗粒细胞层.免疫细胞化学首次在硬骨鱼中证实浦肯野细胞为GABA能细胞，而广突细胞为非GABA能细胞.我们发展了一种用电极和安装在头部的运算放大器记录游泳鱼小脑神经元电活动的方法。根据记录的动作电位形态，成功识别出小脑神经元.希望在此基础上进一步研究，丰富对硬骨鱼类小脑功能的认识。