A micro-stimulation study on the supraspinal descending neurons underlying fish swimming.

鱼类游泳的脊髓上下降神经元的微刺激研究。

• 批准号: 05660209
• 负责人: UEMATSU Kazumasa
• 金额: $ 1.41万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660209/
• 关键词: teleost; carp; swimming locomotion; spinal cord; electrical stimulation; L-glutamate; HRP; brain nucleus; 脳; 運動誘発領域; 脊髄下降路; 微小刺激; 魚類

1.A stereotaxic apparatus for the rat was reconstructed to be usable for small fish by some modifications such as an addition of a gill-irrigating tube.2.The whole mid-brain region of the carp was micro-stimulated with negative rectangular current of 100muA,8msec, 100Hz, or lower. Swimming movement appeared normal was elicited by a stimulation with near threshold currents to the nucleus fasciculus longitudinalis medialis (Nfl), the fasciculus longitudinalis medialis (flm), and the brachium conjunctivum.3.Solution of an excitatory aminoacid, L-glutamate was micro-injected into the vicinity of Nfl. Tail movements beating toward the ipsilateral side were elicited in the most case. Symmetrical tail beats were induced by injections to the middle place of the both Nfls. No swimming occurred by injection to the nucleus ruber.4.HRP injections to flm of the one side labeled neurons in ipsilateral Nfl.5.These results confirm that it is Nfl neurons that send an signal activating the central pattern generators in the spinal cord through flm of the ipsilateral side.6.When Nfl or flm of the one side was destroved by an electric lesion, the fish could not use the axial muscles of the same side. Destruction of the nucleus ruber or the decussatio branchium conjunctivum caused abnormal movements in paired fins.7.As the next step of the investigation, I must examine relationships between each Nfl neuron and segmental pattern generators in the cord, architecture of the network and the control of the midbrain locomotor region by higher brain regions.

2.用100微安、8毫秒、100赫兹或更低的负矩形电流微刺激鲤鱼的整个中脑区。用接近阈值的电流刺激内侧束核(NFL)、内侧束核(Flm)和结臂结膜，可引起正常的游泳运动。3.将兴奋性氨基酸的溶液L-谷氨酸微量注入NFL附近。大多数情况下可引起尾部向同侧跳动。双侧NFL中部注射诱发对称性尾部搏动。4.将HRP注射到同侧NFL标记神经元的延髓内。5.这些结果证实，正是NFL神经元通过同侧延髓发出信号，激活脊髓的中枢模式产生器。6.当一侧NFL或延髓延髓被电损伤时，鱼不能使用同侧的轴肌。破坏红核或结膜十字支会导致成对鳍的异常运动。7.作为下一步的研究，我必须检查每个NFL神经元与脊髓节段性模式生成器之间的关系、网络的结构以及高级脑区对中脑运动区的控制。