Development of therapeutic method which is based on acupuncture induced proliferation of skeletal muscle stem cells

本课题研究了电针刺激骨骼肌干细胞增殖的治疗方法。结果表明，低频电针（30 Hz以下）、中频混合电针和飞秒激光照射对骨骼肌干细胞增殖的刺激效果最好。我们发现在这样的电针和飞秒激光的刺激后没有细胞损伤。对废用性肌萎缩模型小鼠的分析表明，电针可抑制肌肉萎缩，改变肌肉生长抑制素基因表达，降低蛋白丰度，提示AKT/mTOR/FOXO信号通路相关。实验结果还表明，电针不仅能维持肌肉质量，而且能维持肌肉的收缩力

We studied therapeutic method utilizing electro-acupuncture which induces proliferation of skeletal muscle stem cells. Our results showed that effective stimulation condition for the proliferation of skeletal muscle stem cells was electro-acupuncture of the low-frequency up to 30 Hz,that of mixed mid-frequency and the femtosecond laser radiation. We found no cell damages after thestimulation of such electro-acupuncture and the femtosecond laser. Our analysis of the model mouse of disuse muscle atrophy showed that the electro-acupuncture could inhibit muscle atrophy, changed gene expressions, reduced protein abundance of myostatin and the correlation of AKT/mTOR/FOXO signaling was suggested. The result of our experiment also showed that the electro-acupuncture could maintain not only the muscle mass butthe contractile force of muscle