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Construction of molecular basis and clinical application of Kampo medicine

汉方医学的分子基础构建及临床应用

基本信息

批准号：
22590666
负责人：
ARAI Makoto
金额：
$ 2.83万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010-04-01 至 2014-03-31
项目状态：
已结题

项目摘要

Kampo crude drug, daio (Rhei rhizoma) is frequently used as a purgative for a long time. We previously reported the extending life span of nematode treated with daio, however, its biological response by administration of daio remains unclear. In this study, to clarify the molecular mechanisms on life extension by daio in nematode, we screened the genes upregulated by administration of daio using microarray analysis. We obtained six genes transcriptionally activated more than 10 times by daio and their mammalian homologues were characterized in mice. By 2-week oral administration, expression of Nhe1, one of the six candidate genes, in kidney was upregulated similarly to that in nematode. These findings suggest that the Nhe1 encoding Na+/H+ exchanger protein plays an important role in maintaining kidney structurally and functionally.To use daio in animal experiments, we have also developed a method for preparing sennoside-reduced daio, which maintains most of other major components.

汉方药材，daio（甘草）经常被用作泻药很长一段时间。我们先前报道了用daio处理的线虫的寿命延长，然而，通过施用daio的其生物学反应仍不清楚。在这项研究中，为了阐明dao延长线虫寿命的分子机制，我们使用微阵列分析筛选了dao上调的基因。我们获得了六个基因的转录激活超过10倍的daio和他们的哺乳动物同源物的特点是在小鼠。通过2周的口服给药，Nhe 1，6个候选基因之一，在肾脏中的表达上调类似于线虫。这些结果表明，Nhe 1编码的Na+/H+交换蛋白在维持肾脏结构和功能方面起着重要作用。为了在动物实验中使用daio，我们还开发了一种制备番泻苷还原daio的方法，该方法保留了大部分其他主要成分。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Phenolic Compounds and Terpenoids from Hypericum lanceolatum

DOI：
发表时间：
2012
期刊：
Records of Natural Products
影响因子：
1.9
作者：
H. K. Wabo;T. Kowa;A. H. Lonfouo-;Alembert Tchinda Tiabou;P. Tane;H. Kikuchi;M. Frédérich;Y. Oshima
通讯作者：
H. K. Wabo;T. Kowa;A. H. Lonfouo-;Alembert Tchinda Tiabou;P. Tane;H. Kikuchi;M. Frédérich;Y. Oshima

Selective overexpression of Comt in prefrontal cortex rescues schizophrenia-like phenotypes in a mouse model of 22q11 deletion syndrome.

DOI：
10.1038/tp.2012.70
发表时间：
2012-08-07
期刊：
Translational psychiatry
影响因子：
6.8
作者：
Kimoto S;Muraki K;Toritsuka M;Mugikura S;Kajiwara K;Kishimoto T;Illingworth E;Tanigaki K
通讯作者：
Tanigaki K

Xanthones and a benzophenone from the roots of Pentadesma butyracea and their antiproliferative activity