Molecular studies of IG translocation and PVT1 and DCC gene rearrangements in multiple myeloma
Molecular studies of IG translocation and PVT1 and DCC gene rearrangements in multiple myeloma
批准号:
22591045
负责人:
TANIWAKI Masafumi
金额:
$2.91万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2012
中文摘要
为了阐明多发性骨髓瘤(MM)的病理生理,我们使用FISH、SKY、寡核苷酸阵列和RT -PCR方法对染色体8q24和18q21重排的断点进行了分子解剖。54例MM患者中有7例(13.0%)和11例MM细胞系中有5例(45.5%)检测到PVT1重排。鉴定出两个新的嵌合基因PVT1-NBEA和PVT1-WWOX。PVT1外显子1与NBEA外显子2融合的PVT1-NBEA嵌合体和PVT1外显子1与WWOX外显子9融合的PVT1-WWOX嵌合体分别与异常NBEA和缺n端WWOX的表达相关。这些发现表明,PVT1重排可能代表了8q24重排阳性多发性骨髓瘤病理的一种新的分子范式。另一方面,我们对18q21的研究在15个细胞系中发现了DCC异常转录本,其中DCC外显子2与部分DCC内含子1融合,因此缺乏外显子1。推测异常DCC蛋白缺少部分n端,提示DCC结构异常在MM的发生发展中起一定作用。
英文摘要
To elucidate pathophysiology of multiple myeloma (MM), we performed molecular dissection of the breakpoints at chromosomal 8q24 and 18q21 rearrangements using FISH, SKY, oligonucleotide array , and RT -PCR procedures. PVT1 rearrangements were detected in 7 (13.0%) of 54 MM patients and 5 (45.5 %) of 11 MM cell lines. Two novel chimeric genes were identified, PVT1-NBEA and PVT1-WWOX. The PVT1-NBEA chimera in which PVT1 exon 1 was fused to NBEA exon 2 and the PVT1-WWOX in which PVT1 exon 1 was fused to WWOX exon 9 were associated with the expression of abnormal NBEA and WWOX lacking their N-terminus, respectively.These findings suggest that PVT1 rearrangements may represent a novel molecular paradigm underlying the pathology of 8q24 rearrangement-positive multiple myeloma. On the other hand, our studies focusing on 18q21 identified abnormal DCC transcripts in which DCC exon 2 was fused to part of DCC intron 1 hence lacking exon 1 in 15 cell lines using bubble PCR for cDNA. Putative abnormal DCC protein lacks a part of N-terminus, suggesting that structural abnormalities of DCC play some roles in the development and progression of MM.
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RSK2Ser227is a therapeutic target of myeloma cells regardless of upstream signalings
无论上游信号如何,RSK2Ser227 都是骨髓瘤细胞的治疗靶点
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Shimura Y, Kuroda J, Nagoshi H, M Yamamoto-Sugitani M, Kobayashi T, Kiyota M, Nakayama R, Mizutani S, Chinen Y, Sakamoto N, Matsumoto Y, Horiike S, Ri M, Shiotsu Y, Iida S, Taki T, Taniwaki M]
通讯作者:
Taniwaki M
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--
发表时间:
2012
期刊:
影响因子:
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[Rika Kihara, Hitoshi Kiyoi, Takahiro Kato, Erina Yamamoto, Shigeki Ohtake, Shuichi Miyawaki, Yasushi Miyazaki, Norio Asou, Toru Sakura, Yukiyasu Ozawa, Noriko Usui, Heiwa Kanamori, Toru Kiguchi, Kiyotoshi Imai, Naokuni Uike, Fumihiko Kimura, Kunio Kitamur, 古川雄祐, 古林勉]
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古林勉
Low-dose cyclophosphamide (LD-CY)・G-CSF併用による末梢血幹細胞採取(PBSCC)の試み
使用低剂量环磷酰胺 (LD-CY) 和 G-CSF 组合进行外周血干细胞采集 (PBSCC) 的试验
DOI:
--
发表时间:
2012
期刊:
影响因子:
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作者:
[Furukawa, Y., 宮崎泰司, 志村勇司]
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志村勇司
Expression of altered DCC gene in multiple myeloma and non-Hogdgikin's lymphoma
DCC基因改变在多发性骨髓瘤和非霍奇金淋巴瘤中的表达
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Nagoshi H, Taki T, Kuroda J, Nishida K, Gotoh M, Okuda K, Kobayashi S, Yamamoto M, Tsutsumi Y, Kobayashi T, Matsumoto Y, Horiike S, Otsuki T, Taniwaki M]
通讯作者:
Taniwaki M
染色体8q24領域のHSR様増幅により,c-MYCおよびPVT1の同時増幅を認めたDLBCLの一例
染色体8q24区域HSR样扩增导致c-MYC和PVT1同时扩增的DLBCL一例
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[Gokoh M, Nakamura N, Matsuyama S, Nishio M, Akutsu H, Umezawa A, Yasuda K, Yuo A, Saeki K, 山本正樹,西村純一,細川晃平,杉盛千春,米村雄士,小原直,中村嘉彦,野地秀義,七島勉,安藤潔,二宮治彦,千葉滋,川口辰哉,中尾眞二,金倉譲, 水野昌平]
通讯作者:
水野昌平
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Establishment of nanocell fabrication on semiconductor surface utilizing self-organizational movement of point defects
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批准号:22360268
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.56万
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财政年份:2010
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负责人:TANIWAKI Masafumi
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依托单位:
Genomic analysis of multiple myeloma using SKY and oligonucleotide array
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批准号:19591127
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.91万
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财政年份:2007
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负责人:TANIWAKI Masafumi
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依托单位:
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批准号:14570991
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批准号:14350343
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.41万
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财政年份:2002
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负责人:TANIWAKI Masafumi
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依托单位:
SUBCLASSFICIATION OF MULTIPLE MYELOMA BASED ON THE PARTNER GENES OF TUMOR-SPECIFIC IMMUNOGLOBULIN HEAVY CHAIN GENE TRANSLOCATION BY USING DOUBLE-COLOR FLUORESCENCE IN SITU HYBRIDIZATION
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批准号:12671001
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.98万
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财政年份:2000
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负责人:TANIWAKI Masafumi
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INTERPHASE DETECTION OF GENE REARRANGEMENTS SPECIFIC TO NON-HODGKIN'S LYMPHOMA USING DOUBLE-COLOR FISH
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批准号:10670962
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项目类别:Grant-in-Aid for Scientific Research (C)
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负责人:TANIWAKI Masafumi
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依托单位:
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