Regeneration of small intestine with ability of absorption and peristalsis using amniotic membrane and amniotic stem cells

为了克服肠上皮组织再生困难这一具有消化、吸收和蠕动功能的全圆壁肠再生最严重的问题，我们利用羊膜和羊膜干细胞，它们具有良好的诱导细胞和组织再生或多能性分化的能力。在体外实验中，羊膜培养或与羊膜干细胞共培养均能诱导肠上皮细胞的生长和分化。然而，在使用动物模型重建肠道的体内实验中，虽然识别出平滑肌样组织，但肠上皮组织未再生。

To overcome the difficulty of regeneration of intestinal epithelial tissues which is the most serious problems for regeneration of fully circular walled intestine with digestion, absorption and peristalsis, we utilized amniotic membrane and amniotic stem cells, which have excellent ability to induce cell and tissue regeneration or pluripotency to differentiate. In the in vitro analysis, the culture on amniotic membrane or co-culture with amniotic stem cells induced the growth and differentiation of the intestinal epithelial cells. However, in the in vivo experiment to reconstruct the intestine using animal models, the intestinal epithelial tissues were not regenerated although the smooth muscle-like tissues were recognized.