The analysis of liver-to-pancreas reprogramming based on cellular interaction

基于细胞相互作用的肝胰重编程分析

• 批准号: 22591516
• 负责人: KOBAYASHI Akira
• 金额: $ 3.08万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591516/
• 关键词: 再生医療; 遺伝子治療; 細胞間相互作用; リプログラミング

We introduced Pdx1 and Ngn3 into regenerative liver using non-viral gene transfer technique. Co-expression of Pdx1 and Ngn3 activated the transcription of various islet-related genes, and induced insulin synthesis in the liver. Futhermore, it was suggested that this reprogramming was enhanced by co-expression of triple factors adding v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (MafA).In conclusion, the reprogramming will occur even in vivo without the need for viral vectors. Further investigation might be needed to optimize the combination of transgene.

我们采用非病毒基因转移技术将Pdx 1和Ngn 3导入再生肝。Pdx 1和Ngn 3的共表达激活了各种胰岛相关基因的转录，并诱导肝脏中的胰岛素合成。结果表明，加入v-maf肌腱膜纤维肉瘤癌基因家族蛋白A（MafA）的三因子共表达可增强这种重编程，因此，即使不需要病毒载体，重编程也可在体内发生。可能需要进一步研究以优化转基因组合。

项目成果

Induction of pancreatic β-like cells from hepatic cells.

从肝细胞诱导胰腺β样细胞。

• 发表时间: 2012
• 作者: Linuma N;Miyagawa S.;et al.;本山博章;本山博章;本山博章
• 通讯作者: 本山博章

Induction of pancreatic β-like cells from hepatic cells

从肝细胞诱导胰腺β样细胞

• 发表时间: 2012
• 作者: Linuma N;Miyagawa S.;et al.;本山博章;本山博章;本山博章;本山博章
• 通讯作者: 本山博章

An excellent non-viral procedure to induce liver-to-pancreas reprogramming to complement post-operative pancreatic endocrine dysfunction.

一种出色的非病毒程序，可诱导肝脏至胰腺重新编程，以补充术后胰腺内分泌功能障碍。

• 发表时间: 2010
• 作者: Kobayashi A;Miyagawa S;Miwa S;Nakata T.;中田岳成;中田岳成;Hiroaki Motoyama;Hiroaki Motoyama
• 通讯作者: Hiroaki Motoyama

Adrenomedullin in sinusoidal endothelial cells play protective roles against cold injury of liver

• DOI: 10.1016/j.peptides.2010.01.011
• 发表时间: 2010-05
• 期刊: Peptides
• 影响因子: 3
• 作者: Nobuyoshi Iinuma;T. Sakurai;Akiko Kamiyoshi;Yuka Ichikawa-Shindo;Takuma Arai;T. Yoshizawa;T. Koyama;Ryuichi Uetake;Hisaka Kawate;S. Muto;Y. Tagawa;S. Miyagawa;T. Shindo

Clinicopathological features of hepatitis C virus disease after living donor liver transplantation: relationship with in situ hybridisation data.

活体肝移植后丙型肝炎病毒病的临床病理特征：与原位杂交数据的关系。

• DOI: 10.1097/pat.0b013e32834317ed
• 发表时间: 2011
• 期刊: Pathology
• 影响因子: 4.5
• 作者: Masuda Y;Nakazawa Y;Matsuda K;Sano K;Mita A;Ohno Y;Urata K;Ikegami T;Miwa S;Miyagawa S.
• 通讯作者: Miyagawa S.