Basic research for the comprehensive analysis of selective whitematter injury and the axonal regeneration

实验采用SD大鼠。在全身麻醉下，向一侧内囊立体定向注射内皮素-1(ET-1：0.5μg/μ，L)，造成选择性脑白质损伤，用核磁共振评价选择性脑白质损伤，用APP特异性免疫反应确定轴索损伤。注射后3d至2周，ED1免疫反应增强，表明激活的小胶质细胞迁移并占据白质损伤的损伤部位。成像质谱仪(IMS)全面揭示了磷脂酰胆碱的动态变化，具有巨大的潜力，可以为常规模式下看不见的动态分子变化提供新的见解。将微血管内皮细胞移植到内囊缺血区。微血管内皮细胞移植改善了行为结局，诱导了髓鞘再生。MVEC移植对炎症反应也有抑制作用。阐明微血管内皮细胞改善脑白质缺血性损伤的机制可能为开发脑白质缺血的有效治疗方法提供重要信息。

Sprague-Dawley rats were used. Under general anesthesia endothelin-1(ET-1:0.5μg/μl) was stereotactically injected into unilateral internal capsule to induce selective white matter damage.Selective white matter injury was evaluated by MRI, and particularly axonal injury was confirmed by specific APP immunoreactivity. The increased immunoreactivitiy for ED1 was observed in the lesion from3 days to 2 weeks after injection showing the activated microglia migrated and occupied the lesion of white matter injury. Imaging mass spectrometry (IMS) revealed the dynamicchange of phosphatidylcholines comprehensively IMS has tremendous potentials to provide the new insight in terms of the dynamic molecular changes invisible under conventional modality.The microvascular endothelial cells (MVECs) were prepared from rat cerebra for the cell transplantation. MVECs were transplanted into the ischemic lesion of internal capsule. MVECtransplantation improved the behavioral outcome and induced remyelion. Also the inflammatory response was repressed by MVEC transplantation. Elucidation of the mechanisms by which MVECs ameliorate ischemic damage of the white matter mayprovide important information for the development of effective therapies for white matter ischemia.