Direct injection treatment for intra-parenchymal spinal glioma

C6是一种起源于大鼠脑的胶质母细胞瘤细胞。用替莫唑胺（TMZ）体外处理细胞进行剂量效应研究，发现100μM的TMZ对细胞生长有明显抑制作用。然后评价100μM TMZ对大鼠脑实质是否有毒性作用。当注射速度小于0.25μl/min时，未见脑实质损伤。首先，将C6细胞植入双侧腰椎区皮下，C6细胞植入3天后，将TMZ直接注射到肿瘤内。作为对照，在肿瘤内注射生理盐水代替TMZ。TMZ注射组肿瘤生长受到抑制。然后，将C6细胞注射到右脑皮层下，在C6细胞植入3天后，将TMZ直接注射到肿瘤中。对照大鼠肿瘤内注射生理盐水。与对照组相比，tmz治疗的肿瘤生长明显减慢。

C6 is a glioblstoma cell originated from brain of a rat. The cells were treated by temozolomide (TMZ) in vitro for dose-response study, and the cell growth was definitely suppressed in 100μM of TMZ. And next, we evaluated that whether 100μM TMZ causes toxic effect or not to rat's brain parenchyma. No brain parenchymal damage was identified in the brain as far as inject-speed was kept in less than 0.25μl/minutes. First, C6 cells were implanted into subcutaneous in bilateral lumbar regions, and TMZ was injected directly into the tumor 3 days after the implantation of C6 cells. As a control, saline solution was injected into the tumor instead of TMZ. Tumor growth was suppressed in TMZ injected cases. And next, C6 cells were injected into the right brain sub-cortex, and TMZ was injected directly into the tumor 3 days after the implantation of C6 cells.Saline solution was injected into the tumor in control rats. Compared with control,TMZ-treated tumor showed much slower growth.