Direct injection treatment for intra-parenchymal spinal glioma

C6是来源于大鼠脑的胶质母细胞瘤细胞。替莫唑胺（TMZ）体外处理细胞进行剂量反应研究，100μM TMZ明显抑制细胞生长。其次，我们评价了100μM TMZ对大鼠脑实质是否产生毒性作用。只要注射速度保持在0.25μl/min以下，则在脑中未发现脑实质损伤。首先，将C6细胞植入双侧腰部皮下，并在C6细胞植入后3天将TMZ直接注射到肿瘤中。作为对照，将盐水溶液注射到肿瘤中而不是TMZ。TMZ注射病例中肿瘤生长受到抑制。然后将C6细胞植入大鼠右侧大脑皮层下，并于C6细胞植入后第3天将TMZ直接注射到肿瘤内，对照组则将生理盐水注射到肿瘤内。与对照组相比，TMZ治疗的肿瘤生长缓慢得多。

C6 is a glioblstoma cell originated from brain of a rat. The cells were treated by temozolomide (TMZ) in vitro for dose-response study, and the cell growth was definitely suppressed in 100μM of TMZ. And next, we evaluated that whether 100μM TMZ causes toxic effect or not to rat's brain parenchyma. No brain parenchymal damage was identified in the brain as far as inject-speed was kept in less than 0.25μl/minutes. First, C6 cells were implanted into subcutaneous in bilateral lumbar regions, and TMZ was injected directly into the tumor 3 days after the implantation of C6 cells. As a control, saline solution was injected into the tumor instead of TMZ. Tumor growth was suppressed in TMZ injected cases. And next, C6 cells were injected into the right brain sub-cortex, and TMZ was injected directly into the tumor 3 days after the implantation of C6 cells.Saline solution was injected into the tumor in control rats. Compared with control,TMZ-treated tumor showed much slower growth.