Analysis of oncogenes involved in invasion or metastasis of endometrial cancer and detection of endometrial cancer stem cells

应用临床标本对TSC403基因的表达进行分析。在子宫内膜癌组织中观察到T端高表达。没有基因扩增。利用表达TSC403基因的细胞系对肿瘤细胞进行了体外侵袭性分析。相关性显著。从子宫内膜癌组织样本和正常子宫内膜组织样本中，我们使用iTRAQ技术鉴定出表达减弱或增强的蛋白。蛋白X与抗癌药物敏感性有关。肿瘤干细胞的分析采用流式细胞术，使用CD133作为干细胞表面标记物。肿瘤干细胞原代培养完成，但未继续。从子宫内膜癌组织出发，尝试了新的癌细胞调节方法CTOS：癌组织源性球体。它成功地在子宫内膜癌中创造了培养细胞（CTOS）。制备CTOS，可进行体外药敏试验。本研究可能有助于建立一种新的子宫内膜癌治疗体系。

Analysis of the expression of the TSC403 genes using clinical specimens was done. T ended high degree of expression was observed in endometrial cancer tissue. There was no gene amplification.Invasion analysis of cancer cells in vitro using the TSC403 gene expressing cell line was done. Significant correlation was found.From endometrial cancer tissue specimens and normal endometrial tissue samples, using the iTRAQ technique, we identified proteins whose expression has been attenuated or e nhanced. Protein X is related to anti-cancer drug sensitivity .The analysis of cancer stem cells was done by FACS flow cytometry using CD133 as a stem cell surface marker. Primary culture of cancer stem cells was done, but not continued.From endometrial cancer tissue, new cancer cells adjusting method CTOS: cancer tissueoriginated spheroid was tried. It succeeds in creating cultured cells (CTOS) in endometrial cancer. CTOS was created, could be performed vitro drug sensitivity test. This study, may contribute to the development of a new treatment system of endometrial cancer.