Development of efficient hematopoietic stem cell differentiation method from human iPS cell

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To understand the molecular basis of hematopoietic differentiation from human induced pluripotent stem cells (hiPSCs), we first performed chemical compound screening using in vitro differentiation system based on embryoid body (EB) formation. From this screen, we found that several TGFβ inhibitors enhanced hematopoietic cell differentiation. As the result, we established an efficient methodfor induction of hematopoietic stem and progenitor cells from hiPSCs. We also evaluated the effects of overexpression of key molecules predicted to support the generation and maturation of HSCs. Among genes tested, we found that SOX17 plays a critical role in priming hemogenic potential of endothelial cells, thereby regulating the hematopoietic development from hiPSCs.