Gene therapy for pathological vascular remodeling targeting bone marrow-derived smooth muscle progenitor cells

针对骨髓源性平滑肌祖细胞的病理性血管重塑的基因治疗

• 批准号: 22791308
• 负责人: MATSUNO Yukihiro
• 金额: $ 2.58万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791308/
• 关键词: 遺伝子治療; 血管リモデリング; 超音波; ナノバブル; 平滑筋前駆細胞; マイクロバブル

We investigated gene therapy for pathological vascular remodeling targeting bone marrow-derived smooth muscle progenitor cells. In vitro study, green fluorescent protein(GFP) gene was transferred to COS-7 cells using sonoporation and the expression was confirmed by Western blotting and fluorescence microscopy. In vivo study, GFP gene was transferred to rat carotid artery and the gene expression was confirmed by fluorescence microscopy. We will prepare nano-bubbles and evaluate the further effects using a functional gene.

我们研究了针对骨髓来源的平滑肌祖细胞的病理性血管重塑的基因疗法。体外研究中，利用声孔法将绿色荧光蛋白（GFP）基因转移至COS-7细胞中，并通过Western blotting和荧光显微镜证实表达。在体内研究中，将GFP基因转移至大鼠颈动脉，并通过荧光显微镜证实基因表达。我们将制备纳米气泡并使用功能基因评估进一步的效果。