Gene therapy for pathological vascular remodeling targeting bone marrow-derived smooth muscle progenitor cells

我们研究了针对骨髓来源的平滑肌前体细胞的病理性血管重塑的基因治疗。体外实验中，将绿色荧光蛋白(GFP)基因导入COS-7细胞，通过Western blotting和荧光显微镜检测证实了GFP基因的表达。在体内实验中，将GFP基因导入大鼠颈动脉，荧光显微镜观察证实了GFP基因的表达。我们将制备纳米气泡，并使用功能基因来评估进一步的效果。

We investigated gene therapy for pathological vascular remodeling targeting bone marrow-derived smooth muscle progenitor cells. In vitro study, green fluorescent protein(GFP) gene was transferred to COS-7 cells using sonoporation and the expression was confirmed by Western blotting and fluorescence microscopy. In vivo study, GFP gene was transferred to rat carotid artery and the gene expression was confirmed by fluorescence microscopy. We will prepare nano-bubbles and evaluate the further effects using a functional gene.