Identification of hydatidiform mole-related microRNAs and their clinical signification

本研究的目的是鉴定血浆中完全葡萄胎（CHMs）的microRNA标记物。通过下一代测序和定量RT-PCR分析，选择hsa-miR-520f、520b、520c-3p和519b-3p作为chms相关的候选microrna。CHMs妊娠患者血浆无细胞has-miR-520f中位浓度显著高于正常妊娠(27,194 copies/ mL vs 13,118 copies/ mL， Mann-Whitney U检验，P=0。002)。在15例CHMs妊娠中，终止妊娠后血浆无细胞has-miR-520f浓度明显低于终止妊娠前（wilcoxon符号秩检验，P< 0.05）。001)。综上所述，hsa-miR-520f是chms相关的microRNA，其血浆浓度可能是完全葡萄胎的分子标志物。

The aim of this study was to identify the microRNA marker of the complete hydatidiform moles(CHMs) in plasma. By next generation sequencing and quantitative RT-PCR analysis, hsa-miR-520f, 520b, 520c-3p and 519b-3p were selected as candidate CHMs-associated microRNAs. The median plasma concentration of cell-free has-miR-520f in CHMs pregnancy was significantly higher than in normal pregnancy(27, 194 copies/ mL vs 13, 118 copies/ mL, Mann-Whitney U test, P=0. 002). In 15 cases of CHMs pregnancy, the plasma concentration of cell-free has-miR-520f after termination of pregnancy was significantly decreased than that before termination of pregnancy(wilcoxon signed rank test, P<0. 001). In conclusion, the hsa-miR-520f is CHMs-related microRNA and its plasma concentration may be possible molecular marker of complete hydatidiform mole.