Measurement of HTLV-1 provirus loads in pregnant women with HTLV-1 carrier

htlv -1基因组的CR引物位于pX区域，而作为内控物的β -珠蛋白的PCR引物位于外显子2。HTLV-1前病毒载量计算公式为[（HTLV-1 pX拷贝数）/(β -珠蛋白拷贝数/2)]× 10000个细胞。1) HTLV-1感染的定量PCR检测有助于孕妇HTLV-1筛查系统的建立。2)分娩后血液样本HTLV-1基因组前病毒载量显著降低。然而，HTLV-1前病毒载量的变化可能不能反映孕妇CD4+CD25+T细胞的变化。3) 6例HTLV-1携带孕妇脐血细胞检出HTLV-1原病毒，提示宫内感染可能是HTLV-1母婴传播感染之一。

CR primers for HTLV-1genome were located at the pX region, while PCR primers for beta-globin as an internal control were on exon 2. HTLV-1 proviral load was calculated by the formula [(HTLV-1 pX copy number)/(beta-globin copy number/2)] x10, 000 cells. 1) Quantitative PCR test of HTLV-1 infection is useful for the HTLV-1 screening system in pregnant women. 2) The proviral load of HTLV-1 genome in blood samples was decreased significantly after delivery. However, the changes of HTLV-1 proviral load may not reflect the changes of CD4+CD25+T cells in pregnant women.3)In six pregnant women with HTLV-1 carrier, HTLV-1 provirus was detected in umbilical blood cells, suggesting that the intrauterine infection may be one of the mother-to-child transmitted infection of HTLV-1.