Measurement of HTLV-1 provirus loads in pregnant women with HTLV-1 carrier

HTLV-1 携带者孕妇 HTLV-1 原病毒载量的测量

• 批准号: 24791712
• 负责人: MIURA Shoko
• 金额: $ 2.66万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791712/
• 关键词: 母子感染; HTLV-1; スクリーニング検査; PCR検査; 胎内感染; 妊娠; ウイルス量; 妊婦; キャリア; スクリーニング; ウエスタンブロット; 判定保留; PCR; 臍帯血

CR primers for HTLV-1genome were located at the pX region, while PCR primers for beta-globin as an internal control were on exon 2. HTLV-1 proviral load was calculated by the formula [(HTLV-1 pX copy number)/(beta-globin copy number/2)] x10, 000 cells. 1) Quantitative PCR test of HTLV-1 infection is useful for the HTLV-1 screening system in pregnant women. 2) The proviral load of HTLV-1 genome in blood samples was decreased significantly after delivery. However, the changes of HTLV-1 proviral load may not reflect the changes of CD4+CD25+T cells in pregnant women.3)In six pregnant women with HTLV-1 carrier, HTLV-1 provirus was detected in umbilical blood cells, suggesting that the intrauterine infection may be one of the mother-to-child transmitted infection of HTLV-1.

HTLV-1基因组的PCR引物位于pX区，而作为内部对照的β-珠蛋白的PCR引物位于外显子2。通过公式[（HTLV-1 pX拷贝数）/（β-珠蛋白拷贝数/2）] × 10，000个细胞计算HTLV-1前病毒载量。1)HTLV-1感染的定量PCR检测可用于孕妇HTLV-1筛查系统。2)分娩后，血液样品中HTLV-1基因组的前病毒载量显著降低。但HTLV-1前病毒载量的变化可能不能反映孕妇体内CD 4 + CD 25 +T细胞的变化。3）6例HTLV-1携带者孕妇脐血细胞中检测到HTLV-1前病毒，提示宫内感染可能是HTLV-1母婴传播感染之一。

项目成果

胎盤機能の網羅的かつ非侵襲的評価方法および検査用試薬

胎盘功能综合无创评估方法及检测试剂

• 发表时间: 2008

Circulating levels of maternal plasma cell-free pregnancy-associated placenta-specific microRNAs are associated with placental weight.

• DOI: 10.1016/j.placenta.2014.06.002
• 发表时间: 2014-10
• 期刊: Placenta
• 影响因子: 3.8
• 作者: K. Miura;Shintaro Morisaki;S. Abe;A. Higashijima;Y. Hasegawa;S. Miura;Seiko Tateishi;Hiroyuki Mishima;K. Yoshiura;H. Masuzaki

Single human papillomavirus 16 or 52 infection and later cytological findings in Japanese women with NILM or ASC-US.

日本 NILM 或 ASC-US 女性中的单一人乳头瘤病毒 16 或 52 感染以及随后的细胞学检查结果。

• DOI: 10.1038/jhg.2014.9
• 发表时间: 2014
• 期刊: Journal of Human Genetics
• 影响因子: 3.5
• 作者: Abe S;Miura K;Kinoshita A;Mishima H;Miura S;Yamasaki K;Hasegawa Y;Higashijima A;Jo O;Yoshida A;Kaneuchi M;Yoshiura K;Masuzaki H.
• 通讯作者: Masuzaki H.

Identification of endometrioid endometrial carcinoma-associated microRNAs in tissue and plasma.

• DOI: 10.1016/j.ygyno.2014.01.029
• 发表时间: 2014-03
• 期刊: Gynecologic oncology
• 影响因子: 4.7
• 作者: Ozora Tsukamoto;K. Miura;H. Mishima;S. Abe;M. Kaneuchi;A. Higashijima;S. Miura;A. Kinoshita;K. Y

Pre-vaccination epidemiology of human papillomavirus infections in Japanese women with abnormal cytology.

细胞学异常的日本女性人乳头瘤病毒感染的疫苗接种前流行病学。

• 发表时间: 2011
• 期刊: The Journal of Obstetrics and Gynaecology Research
• 作者: Yamasaki K;Miura K;Shimada T;Ikemoto R;Miura S;Murakami M;Sameshima T;Fujishita A;Kotera K;Kinoshita A;Yoshiura K;Masuzaki H.
• 通讯作者: Masuzaki H.