The role of transcription factor Tead1 in the differentiation and proliferation of retinal pigment epithelial cells.

我利用在人类Sveinsson‘S脉络膜视网膜萎缩症中发现的一个Tead1突变体，研究了转录因子Tead1在视网膜色素上皮细胞分化和增殖中的作用。在原代培养的小鼠视网膜色素上皮细胞中表达Tead1或其突变体，并分析Tead1及其突变体对细胞增殖和TEAD靶基因表达的影响。此外，我还阐明了TEAD转录因子和共激活因子YAP和TAZ在小鼠胚胎眼睛发育过程中的表达模式。

I studied the role of transcription factor Tead1 in the differentiation and proliferation of retinal pigment epithelial cells by using a mutant of Tead1 identified in human Sveinsson' s chorioretinal atrophy. Tead1 or its mutant was expressed in mouse embryonic primary retinal pigment epithelial cells culture, and the cells were analyzed for the proliferation and the expression of Tead target genes. In addition, I clarified the expression patterns of Tead transcription factors and the coactivators, YAP and TAZ, during eye development in mouse embryos.