Establishment and analysis of cancer stem cell and induced cancer stem cell of refractory pediatric solid organ neoplasm.

难治性小儿实体器官肿瘤肿瘤干细胞及诱导肿瘤干细胞的建立与分析

• 批准号: 22791713
• 负责人: YAMAMOTO Yuki
• 金额: $ 2.5万
• 依托单位: 独立行政法人国立がん研究センター (2011)Keio University (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791713/
• 关键词: EGFR; セツキシマブ; Actinomycin D; 横紋筋肉腫; がん幹細胞; 小児固形腫瘍; iPs

We found that Rhabdomyosarcoma cell line, RD and Rh30 have a large number of EGFR positive cells, whereas KYM-1 and RMS-YM have small number of EGFR positive cells by FACS and real time PCR.The combination of cetuximab and actinomycin D was highly synergistic in inhibiting cell growth with EGFR positive cell line. The combination of two reagents increased rates of apoptosis to EGFR positive cells.

我们发现，横纹肌肉瘤细胞系，RD和RH30具有大量的EGFR阳性细胞，而KYM-1和RMS-ym通过FACS和实时PCR具有少量的EGFR阳性细胞。西替辛基和放线肌动物素D的组合在抑制EGFR阳性细胞细胞生长的高度协同中是高度协同的。两种试剂的组合增加了对EGFR阳性细胞的凋亡率。